Na2SO4 and NaCl salts differentially modulate the antioxidant systems in the highly stress tolerant halophyte Prosopis strombulifera

Abstract

Prosopis strombulifera (Lam.) Benth. is a halophytic shrub abundant in high-salinity areas in central Argentina, with high tolerance against NaCl but strong growth inhibition by Na₂SO₄. In the present study, the modulation of the antioxidant systems (enzymatic and non-enzymatic components) was analyzed under different salt treatments (NaCl, Na₂SO₄ and the iso-osmotic mixture) in hydroponic cultivation. Na₂SO₄-treated plants showed strong indications of oxidative stress (H₂O₂ and O₂-• increase). Modifications in antioxidant enzymes activities were observed mainly under Na₂SO₄ treatment, where CAT seems to play an important role in early detoxification of H₂O₂ in roots, whereas SOD and APX have a predominant role in leaves. As part of the non-enzymatic system, 21 compounds were identified in leaves, being polyphenols the most abundant. Control plants contained the major variety of detected phytochemicals (14). Na₂SO₄-treated plants contained 10 compounds and NaCl-treated plants nine compounds, but with a different profile. NaCl-treated plants showed the highest antioxidant capacity. Our findings confirm that different types of salt treatments provoke a differential modulation of the antioxidant systems. Polyphenols and other ROS-detoxifying compounds, in a joint action with the enzymatic antioxidant system, are proposed to have a fundamental role in the cellular protection of P. strombulifera plants under severe oxidative stress. Our findings also highlight the potential of this halophyte as a valuable source of bioactive compounds with high antioxidant activity and health benefits.

Keywords: Enzymatic antioxidants; Na(2)SO(4); NaCl; Non-enzymatic antioxidants; Oxidative stress; Polyphenols; Reactive oxygen species (ROS).