Prediction of Mannheimia haemolytica serotypes based on whole genomic sequences

Henrik Christensen; Magne Bisgaard; Theresa Menke; Martin Liman; Edouard Timsit; Geoffrey Foster; John E Olsen

doi:10.1016/j.vetmic.2021.109232

Prediction of Mannheimia haemolytica serotypes based on whole genomic sequences

Vet Microbiol. 2021 Nov:262:109232. doi: 10.1016/j.vetmic.2021.109232. Epub 2021 Sep 6.

Authors

Henrik Christensen¹, Magne Bisgaard², Theresa Menke³, Martin Liman³, Edouard Timsit⁴, Geoffrey Foster⁵, John E Olsen⁶

Affiliations

¹ Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Fredriksberg C, Denmark. Electronic address: hech@sund.ku.dk.
² Bisgaard Consulting, Viby Sj, Denmark.
³ Anicon, Hoeltinghausen, Germany.
⁴ Edouard Timsit, Innovation and Development Department, Ceva Santé Animale, 10 avenue de la Ballastière, Libourne, France.
⁵ SRUC Veterinary Service, An Lochran 10, Inverness Campus, Inverness, UK.
⁶ Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Fredriksberg C, Denmark.

PMID: 34509701
DOI: 10.1016/j.vetmic.2021.109232

Abstract

The aim of the investigation was to predict the serotypes of M. haemolytica based on whole genomic sequences with the capsular gene region as target. A total of 22 strains selected to have been serotyped and to represent all serotypes were investigated by whole genomic sequencing. The BIGSdb (Bacterial Isolate Genome Sequence Database) was downloaded and installed on a Linux server. Here the sequence database was setup with unique loci at serotype level. The server allows serotypes of M. haemolytica to be predicted from whole genomic sequences and the service is available to the public for free from https://ivsmlst.sund.root.ku.dk.

Keywords: Bovine respiratory disease; Capsular gene region; Indirect haemagglutination; MLST; Non-typeable.

MeSH terms

Animals
Genome, Bacterial*
Genomics
Mannheimia haemolytica* / classification
Mannheimia haemolytica* / genetics
Serogroup
Whole Genome Sequencing / veterinary