Transcriptome analysis of low-temperature-affected ripening revealed MYB transcription factors-mediated regulatory network in banana fruit

Yingying Yang; Mengge Jiang; Jintao Feng; Chaojie Wu; Wei Shan; Jianfei Kuang; Jianye Chen; Zhuoyan Hu; Wangjin Lu

doi:10.1016/j.foodres.2021.110616

Transcriptome analysis of low-temperature-affected ripening revealed MYB transcription factors-mediated regulatory network in banana fruit

Food Res Int. 2021 Oct:148:110616. doi: 10.1016/j.foodres.2021.110616. Epub 2021 Jul 27.

Authors

Yingying Yang¹, Mengge Jiang², Jintao Feng², Chaojie Wu², Wei Shan², Jianfei Kuang², Jianye Chen², Zhuoyan Hu³, Wangjin Lu⁴

Affiliations

¹ State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Engineering Research Center of Southern Horticultural Products Preservation, Ministry of Education, College of Horticulture, South China Agricultural University, Guangzhou 510642, China; College of Food Science, South China Agricultural University, Guangzhou 510642, China.
² State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Engineering Research Center of Southern Horticultural Products Preservation, Ministry of Education, College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
³ College of Food Science, South China Agricultural University, Guangzhou 510642, China. Electronic address: zyhu@scau.edu.cn.
⁴ State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Engineering Research Center of Southern Horticultural Products Preservation, Ministry of Education, College of Horticulture, South China Agricultural University, Guangzhou 510642, China. Electronic address: wjlu@scau.edu.cn.

PMID: 34507760
DOI: 10.1016/j.foodres.2021.110616

Abstract

Low temperature leads to abnormal ripening and poor quality of the harvested banana fruit, which is an urgent problem limiting the development of industry in China. To comprehensively understand the mechanism underlying low-temperature-affected ripening, we performed comparative RNA-Seq analysis of ethylene-induced ripening of banana fruit after 3 days of pre-storage at 7 °C and 22 °C. A total of 986 differentially expressed genes (DEGs) were identified in both RT-0 d versus RT-3 d, LT-0 d versus LT-3 d, RT-0 d versus LT-0 d and RT-3 d versus LT-3 d, and the RNA-Seq outputs of 15 randomly selective DEGs were verified using qRT-PCR. Among the 986 DEGs obtained in the four groups, 9 MYB genes (MaMYB75/281/219/4/151/156/3/37 and MaMYB3R1) and 32 genes related to carotenoid biosynthesis (MaPSY1/2a), flavor formation (MaLOX6, MaADH7, MaAAT1), sucrose transport (MaSUS2/4), ethylene production (MaSAM1, MaACO9/10/12, MaACS1/12), starch degradation (MaAMY1A/1B, MaPHS1/2, MaMEX2, MapGlcT1) and cell wall degradation (MaPG3/X1, MaPME25/41, MaXTH5/7/22/23/25, MaEXP2/20/A1/A15) were characterized. Combining transcription factor binding site (TFBS) analysis as well as cis-acting element analysis, the regulatory network of low-temperature-affected ripening mediated by MYBs were constructed. The data generated in this study may unravel the transcriptional regulatory network of MYBs associated with low-temperature-affected ripening and provide a solid foundation for future studies.

Keywords: Banana; Fruit; Low-temperature-affected ripening; MYBs; Regulatory network; Transcriptome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Fruit / genetics
Fruit / metabolism
Gene Expression Profiling
Gene Expression Regulation, Plant
Musa* / genetics
Musa* / metabolism
Plant Proteins / genetics
Plant Proteins / metabolism
Temperature
Transcription Factors / genetics

Substances

Plant Proteins
Transcription Factors