Listeria monocytogenes biofilm inhibition on food contact surfaces by application of postbiotics from Lactobacillus curvatus B.67 and Lactobacillus plantarum M.2

Md Iqbal Hossain; Md Furkanur Rahaman Mizan; Pantu Kumar Roy; Shamsun Nahar; Sazzad Hossen Toushik; Md Ashrafudoulla; Iqbal Kabir Jahid; Jihyun Lee; Sang-Do Ha

doi:10.1016/j.foodres.2021.110595

Listeria monocytogenes biofilm inhibition on food contact surfaces by application of postbiotics from Lactobacillus curvatus B.67 and Lactobacillus plantarum M.2

Food Res Int. 2021 Oct:148:110595. doi: 10.1016/j.foodres.2021.110595. Epub 2021 Jul 10.

Authors

Md Iqbal Hossain¹, Md Furkanur Rahaman Mizan¹, Pantu Kumar Roy¹, Shamsun Nahar¹, Sazzad Hossen Toushik¹, Md Ashrafudoulla¹, Iqbal Kabir Jahid², Jihyun Lee³, Sang-Do Ha⁴

Affiliations

¹ Department of Food Science and Technology, Advanced Food Safety Research Group, Chung-Ang University, 72-1 Nae-Ri, Daedeok-Myun, Anseong, Gyunggido 456-756, Republic of Korea.
² Department of Microbiology, Jashore University of Science and Technology, Bangladesh.
³ Department of Food Science and Technology, Chung-Ang University, Anseong 17546, Republic of Korea.
⁴ Department of Food Science and Technology, Advanced Food Safety Research Group, Chung-Ang University, 72-1 Nae-Ri, Daedeok-Myun, Anseong, Gyunggido 456-756, Republic of Korea. Electronic address: sangdoha@cau.ac.kr.

PMID: 34507740
DOI: 10.1016/j.foodres.2021.110595

Abstract

Owing to their preservative and antimicrobial effects, postbiotics (metabolic byproducts of probiotics) are promising natural components for the food industry. Therefore, the present study aimed to investigate the efficacy of postbiotics collected from isolated Lactobacillus curvatus B.67 and Lactobacillus plantarum M.2 against Listeria monocytogenes pathogens in planktonic cells, motility, and biofilm states. The analysis of the metabolite composition of the postbiotics revealed various organic acids, along with a few well-known bacteriocin-encoding genes with potential antimicrobial effects. Postbiotics maintained their residual antimicrobial activity over the pH range 1-6 but lost all activity at neutral pH (pH 7). Full antimicrobial activity (100%) was observed during heat treatment, even under the autoclaving condition.Minimum inhibitory concentration (MICs) of L. curvatus B.67 and L. plantarum M.2 against L. monocytogenes were 80 and 70 mg/mL, respectively. However, four sub-MICs of the postbiotics (1/2, 1/4, 1/8, and 1/16 MIC) were tested for inhibition efficacy against L. monocytogenes during different experiment in this study. Swimming motility, biofilm formation, and expression levels of target genes related to biofilm formation, virulence, and quorum-sensing were significantly inhibited with increasing postbiotics concentration. Postbiotics from L. plantarum M.2 exhibited a higher inhibitory effect than the postbiotics from L. curvatus B.67. Nonetheless, both these postbiotics from Lactobacillus spp. could be used as effective bio-interventions for controlling L. monocytogenes biofilm in the food industry.

Keywords: Biofilm; Food contact surface; Food industry; Listeria monocytogenes; Metabolite; Postbiotic; Probiotic.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biofilms
Lactobacillus
Lactobacillus plantarum*
Listeria monocytogenes*

Supplementary concepts

Lactobacillus curvatus