Immunohistochemical and Morphological Features of Bovine Granulosa Cell Tumours in Relation to Growth Pattern and Folliculogenesis

Angeline P P Teh; Go Kitahara; Uda Z Izzati; Keita Mori; Naoyuki Fuke; Takuya Hirai; Ryoji Yamaguchi

doi:10.1016/j.jcpa.2021.06.008

Immunohistochemical and Morphological Features of Bovine Granulosa Cell Tumours in Relation to Growth Pattern and Folliculogenesis

J Comp Pathol. 2021 Aug:187:40-51. doi: 10.1016/j.jcpa.2021.06.008. Epub 2021 Aug 3.

Authors

Angeline P P Teh¹, Go Kitahara², Uda Z Izzati¹, Keita Mori¹, Naoyuki Fuke¹, Takuya Hirai¹, Ryoji Yamaguchi³

Affiliations

¹ Department of Veterinary Pathology.
² Laboratory of Theriogenology, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
³ Department of Veterinary Pathology. Electronic address: a0d402u@cc.miyazaki-u.ac.jp.

PMID: 34503653
DOI: 10.1016/j.jcpa.2021.06.008

Abstract

Granulosa cell tumours (GCTs) are the most common type of bovine ovarian neoplasm. We describe the morphological and immunohistochemical features of GCTs (n = 26) and a thecoma (n = 1) using antibodies to cytokeratin AE1/AE3, vimentin, desmin, nectin-4, anti-Müllerian hormone (AMH), inhibin α, c-erbB-2 oncoprotein and proliferating cell nuclear antigen. The results indicate that, based on the expression similarity of AE1/AE3 and inhibin α, the macrofollicular growth pattern of GCTs derives from highly differentiated granulosa cells (GCs), the Graafian follicle or early atretic follicle. The trabecular growth pattern of GCTs is inferred to be derived from GCs of the atretic follicle in mature animals. AMH is a useful marker for diagnosing bovine sex cord-stromal tumours because it was expressed in all GCT cases and in the single case of thecoma.

Keywords: anti-Müllerian hormone; granulosa cell tumour; immunohistochemistry; thecoma.

MeSH terms

Animals
Cattle
Cattle Diseases*
Female
Granulosa Cell Tumor* / veterinary
Ovarian Follicle
Ovarian Neoplasms* / veterinary
Sex Cord-Gonadal Stromal Tumors* / veterinary