Graphene functionalized with structurally complementary amino acids for sensitive recognition of N-linked glycopeptides

Canhong Zhu; Jiani Wu; Xueting Jin; Yinghua Yan; Chuan-Fan Ding; Keqi Tang; Qiaohong Zhang

doi:10.1016/j.chroma.2021.462505

Graphene functionalized with structurally complementary amino acids for sensitive recognition of N-linked glycopeptides

J Chromatogr A. 2021 Oct 11:1655:462505. doi: 10.1016/j.chroma.2021.462505. Epub 2021 Sep 2.

Authors

Canhong Zhu¹, Jiani Wu¹, Xueting Jin¹, Yinghua Yan², Chuan-Fan Ding³, Keqi Tang¹, Qiaohong Zhang⁴

Affiliations

¹ Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, School of Materials Science and Chemical Engineering, Institute of Mass Spectrometry, Ningbo University, Ningbo 315211, China.
² Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, School of Materials Science and Chemical Engineering, Institute of Mass Spectrometry, Ningbo University, Ningbo 315211, China. Electronic address: yanyinghua@nbu.edu.cn.
³ Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, School of Materials Science and Chemical Engineering, Institute of Mass Spectrometry, Ningbo University, Ningbo 315211, China. Electronic address: dingchuanfan@nbu.edu.cn.
⁴ Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, School of Materials Science and Chemical Engineering, Institute of Mass Spectrometry, Ningbo University, Ningbo 315211, China. Electronic address: zhangqiaohong@nbu.edu.cn.

PMID: 34500222
DOI: 10.1016/j.chroma.2021.462505

Abstract

Herein, a hydrophilic graphene composite functionalized with glutathione (GSH) and L(+)-Cysteine (Cys) was prepared via a simple and fast synthesis route, which was named G@S@Au@GC. The combination attack with two different zwitterionic polymers resulted in enhanced adsorption sites for glycopeptides. The obtained G@S@Au@GC exhibited excellent performance on a low limit of detection (0.2 fmol), a high selectivity (HRP: bovine serum albumin = 1:1500), a good load capacity (250 μg•mg^-1) and recovery rate (93%), which was also evaluated with IgG. Subsequently, 60 glycopeptides from complex biological sample (human saliva) were identified by Nano-LC-MS/MS. The advantages of combination attack, low-cost, simple and fast synthesis, and superior enrichment performance make G@S@Au@GC composite a bright future on glycoproteomics analysis and related diseases.

Keywords: Au nanoparticles; G@S@Au@GC; Glycopeptides enrichment; MALDI-TOF mass spectrometry; Zwitterionic polymer.

MeSH terms

Amino Acids
Glycopeptides*
Graphite*
Humans
Hydrophobic and Hydrophilic Interactions
Tandem Mass Spectrometry

Substances

Amino Acids
Glycopeptides
Graphite