Electrochemical detection of matrix metalloproteinase-7 using an immunoassay on a methylene blue/2D MoS2/graphene oxide electrode

Patrawadee Yaiwong; Natthawat Semakul; Suwussa Bamrungsap; Jaroon Jakmunee; Kontad Ounnunkad

doi:10.1016/j.bioelechem.2021.107944

Electrochemical detection of matrix metalloproteinase-7 using an immunoassay on a methylene blue/2D MoS₂/graphene oxide electrode

Bioelectrochemistry. 2021 Dec:142:107944. doi: 10.1016/j.bioelechem.2021.107944. Epub 2021 Aug 28.

Authors

Patrawadee Yaiwong¹, Natthawat Semakul², Suwussa Bamrungsap³, Jaroon Jakmunee⁴, Kontad Ounnunkad⁵

Affiliations

¹ Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; The Graduate School, Chiang Mai University, Chiang Mai 50200, Thailand; Center of Excellence for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
² Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Research Center on Chemistry for Development of Health Promoting Products from Northern Resources, Chiang Mai University, Chiang Mai 50200, Thailand; Center of Excellence in Materials Science and Technology, Chiang Mai University, Chiang Mai 50200, Thailand.
³ National Nanotechnology Center (NANOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand.
⁴ Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Center of Excellence for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Research Center on Chemistry for Development of Health Promoting Products from Northern Resources, Chiang Mai University, Chiang Mai 50200, Thailand.
⁵ Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Center of Excellence for Innovation in Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; Research Center on Chemistry for Development of Health Promoting Products from Northern Resources, Chiang Mai University, Chiang Mai 50200, Thailand; Center of Excellence in Materials Science and Technology, Chiang Mai University, Chiang Mai 50200, Thailand. Electronic address: kontad.ounnunkad@cmu.ac.th.

PMID: 34500138
DOI: 10.1016/j.bioelechem.2021.107944

Abstract

Methylene blue (MB) adsorption onto a two-dimensional molybdenum disulfide (2D MoS₂)/graphene oxide (GO) nanocomposite sitting on a screen-printed carbon electrode (SPCE) is used to develop a new sensitive label-free electrochemical immunosensor for the detection of matrix metalloproteinase-7 (MMP-7) cancer biomarkers. The 2D MoS₂/GO nanocomposite deposited onto an SPCE provides a large specific surface area, fast electron transfer, and exceptional electrical conductivity. Furthermore, MB adsorbed onto the 2D MoS₂/GO nanocomposite architecture can be used for signal amplification in electrochemical immunosensors. Moreover, an immunosensor platform was fabricated by the adsorption of anti-MMP-7 capture antibodies onto the MB/2D MoS₂/GO nanocomposite surface via electrostatic interactions for the detection of the MMP-7 immunocomplex. Under optimum conditions, the label-free immunosensor exhibits a decrease in the current response for MB corresponding to the MMP-7 concentration. The sensor affords a linear logarithmic range of 0.010-75 ng mL^-1 with a limit of detection (LOD) of 0.007 ng mL^-1. The developed electrochemical immunosensor provides high selectivity, good reproducibility, and excellent stability. Furthermore, the proposed immunosensor can be applied for the detection of MMP-7 in human serum samples with good recovery. Thus, this device can be applied for the early clinical diagnosis of pancreatic and colorectal cancers.

Keywords: 2D MoS(2); Cancer; Electrochemical immunosensor; Graphene oxide; Label-free detection; Matrix metalloproteinase-7.

MeSH terms

Biosensing Techniques / methods*
Disulfides / chemistry
Electrochemical Techniques / methods*
Graphite / chemistry
Humans
Immunoassay / methods*
Matrix Metalloproteinase 7 / blood*
Methylene Blue / chemistry
Molybdenum / chemistry

Substances

Disulfides
graphene oxide
Graphite
Molybdenum
MMP7 protein, human
Matrix Metalloproteinase 7
Methylene Blue
molybdenum disulfide