A Novel, Small Cysteine-Rich Effector, RsSCR10 in Rhizoctonia solani Is Sufficient to Trigger Plant Cell Death

Xianyu Niu; Guijing Yang; Hui Lin; Yao Liu; Ping Li; Aiping Zheng

doi:10.3389/fmicb.2021.684923

A Novel, Small Cysteine-Rich Effector, RsSCR10 in Rhizoctonia solani Is Sufficient to Trigger Plant Cell Death

Front Microbiol. 2021 Aug 23:12:684923. doi: 10.3389/fmicb.2021.684923. eCollection 2021.

Authors

Xianyu Niu¹, Guijing Yang², Hui Lin¹, Yao Liu^{2

3}, Ping Li^{2

3}, Aiping Zheng^{1

2

3}

Affiliations

¹ College of Agronomy, Sichuan Agricultural University, Chengdu, China.
² Rice Research Institute, Sichuan Agricultural University, Chengdu, China.
³ Key Laboratory of Sichuan Crop Major Disease, Sichuan Agricultural University, Chengdu, China.

Abstract

The necrotrophic phytopathogen Rhizoctonia solani (R. solani) is a fungus that causes disease in a wide range of plant species. Fungal genomes encode abundant, small cysteine-rich (SCR) secreted proteins, and the probable importance of these to pathogenesis has been highlighted in various pathogens. However, there are currently no reports of an R. solani SCR-secreted protein with evidential elicitor activity. In this study, the molecular function of 10 SCR-secreted protein genes from R. solani was explored by agroinfiltration into Nicotiana benthamiana (N. benthamiana) leaves, and a novel SCR protein RsSCR10 was identified that triggered cell death and oxidative burst in tobacco. RsSCR10 comprises 84 amino acids, including a signal peptide (SP) of 19 amino acids that is necessary for RsSCR10 to induce tobacco cell death. Elicitation of cell death by RsSCR10 was dependent on Hsp90 but not on RAR1, proving its effector activity. Two cysteine residues have important effects on the function of RsSCR10 in inducing cell death. Furthermore, RsSCR10 showed cross-interaction with five rice molecules, and the inferred functions of these rice proteins suggest they are instrumental in how the host copes with adversity. Overall, this study demonstrates that RsSCR10 is a potential effector that has a critical role in R. solani AG1 IA-host interactions.

Keywords: N. benthamiana; R. solani AG1IA; SCR secreted protein; cell death; immune response; protein interaction.