Genome-Wide Identification and Validation of Target Genes Associated with Insecticide Treatment of the Green Peach Aphid, Myzus persicae

Sadia Iqbal; John Fosu-Nyarko; Frances Brigg; Michael G K Jones

doi:10.1007/978-1-0716-1633-8_11

Genome-Wide Identification and Validation of Target Genes Associated with Insecticide Treatment of the Green Peach Aphid, Myzus persicae

Methods Mol Biol. 2022:2360:119-138. doi: 10.1007/978-1-0716-1633-8_11.

Authors

Sadia Iqbal^{1

2}, John Fosu-Nyarko^{3

4}, Frances Brigg², Michael G K Jones^{1

2}

Affiliations

¹ Crop Biotechnology Research Group, College of Science, Health, Engineering and Education, Murdoch University, Perth, WA, Australia.
² Western Australian State Agricultural Biotechnology Centre, College of Science, Health, Engineering and Education, Murdoch University, Perth, WA, Australia.
³ Crop Biotechnology Research Group, College of Science, Health, Engineering and Education, Murdoch University, Perth, WA, Australia. J.Fosu-Nyarko@murdoch.edu.au.
⁴ Western Australian State Agricultural Biotechnology Centre, College of Science, Health, Engineering and Education, Murdoch University, Perth, WA, Australia. J.Fosu-Nyarko@murdoch.edu.au.

PMID: 34495512
DOI: 10.1007/978-1-0716-1633-8_11

Abstract

Next-generation sequencing and analyses of whole-genome transcripts can be used to identify genes and potential mechanisms that may be responsible for the development of resistance to insecticides. Such genes can be identified by isolating and sequencing high-quality messenger RNA and identifying differentially expressed genes (DEGs), and gene variants from insecticide-treated and untreated colonies of the Green peach aphid (GPA) or resistant and susceptible GPA populations. Datasets generated would reveal a set of genes whose expression may be associated with the insecticide treatment. The DEGs can then be validated using quantitative PCR assays.

Keywords: Differential gene expression; Differentially expressed genes (DEGs); Insecticide resistance; Insects; Next-generation sequencing; RNA-seq; Transcriptome; Whole-genome transcriptome.

MeSH terms

Animals
Aphids* / genetics
Insecticide Resistance / genetics
Insecticides / toxicity
Prunus persica* / drug effects
Prunus persica* / genetics

Substances

Insecticides