Autophagy protects murine preputial glands against premature aging, and controls their sebum phospholipid and pheromone profile

Heidemarie Rossiter; Dragan Copic; Martin Direder; Florian Gruber; Samuele Zoratto; Martina Marchetti-Deschmann; Christopher Kremslehner; Michaela Sochorová; Ionela-Mariana Nagelreiter; Veronika Mlitz; Maria Buchberger; Barbara Lengauer; Bahar Golabi; Supawadee Sukseree; Michael Mildner; Leopold Eckhart; Erwin Tschachler

doi:10.1080/15548627.2021.1966716

Autophagy protects murine preputial glands against premature aging, and controls their sebum phospholipid and pheromone profile

Autophagy. 2022 May;18(5):1005-1019. doi: 10.1080/15548627.2021.1966716. Epub 2021 Sep 7.

Authors

Heidemarie Rossiter¹, Dragan Copic², Martin Direder², Florian Gruber¹, Samuele Zoratto³, Martina Marchetti-Deschmann³, Christopher Kremslehner¹, Michaela Sochorová¹, Ionela-Mariana Nagelreiter^{1

4}, Veronika Mlitz¹, Maria Buchberger¹, Barbara Lengauer¹, Bahar Golabi¹, Supawadee Sukseree¹, Michael Mildner¹, Leopold Eckhart¹, Erwin Tschachler¹

Affiliations

¹ Department of Dermatology, Medical University of Vienna, Vienna, Austria.
² Laboratory for Cardiac and Thoracic Diagnosis, Regeneration and Applied Immunology, Department of Thoracic Surgery, Medical University of Vienna, Vienna, Austria.
³ Institute of Chemical Technologies and Analytics, Technical University of Vienna, Vienna, Austria.
⁴ Center for Brain Research, Department of Molecular Neurosciences, Medical University of Vienna, Vienna, Austria.

Abstract

ATG7: autophagy related 7; BODIPY: boron dipyrromethene; DAG: diacyl glycerides; DBI: diazepam binding inhibitor; GFP: green fluorescent protein; KRT14: keratin 14; HPLC-MS: high performance liquid chromatography-mass spectrometry; LD: lipid droplet; MAP1LC3/LC3: microtubule-associated protein 1 light chain 3; MSI: mass spectrometric imaging; ORO: Oil Red O; PC: phosphatidylcholine; PE: phosphatidylethanolamine; PG: preputial gland; PLIN2: perilipin 2; PtdIns: phosphatidylinositol; PL: phospholipids; POPC: 1-palmitoyl-2-oleoyl-PC; PS: phosphatidylserine; qRT-PCR: quantitative reverse transcribed PCR; SG: sebaceous gland; scRNAseq: single-cell RNA sequencing; TAG: triacylglycerides; TLC: thin layer chromatography.

Keywords: Autophagosomes; GFP-LC3; lipid droplets; perilipin; sebocytes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aging, Premature*
Animals
Autophagy / genetics
Mice
Perilipin-2
Pheromones
Phosphatidylserines
Phospholipids
Sebum*

Substances

Perilipin-2
Pheromones
Phosphatidylserines
Phospholipids