Citrus Segment Degradation Potential, Enzyme Safety Evaluation, and Whole Genome Sequence of Aspergillus aculeatus Strain ZC-1005

Jieyi Wang; Zhipeng Gao; Yujiao Qian; Xiao Hu; Gaoyang Li; Fuhua Fu; Jiajing Guo; Yang Shan

doi:10.3389/fmicb.2021.671200

Citrus Segment Degradation Potential, Enzyme Safety Evaluation, and Whole Genome Sequence of Aspergillus aculeatus Strain ZC-1005

Front Microbiol. 2021 Aug 20:12:671200. doi: 10.3389/fmicb.2021.671200. eCollection 2021.

Authors

Jieyi Wang¹, Zhipeng Gao², Yujiao Qian³, Xiao Hu¹, Gaoyang Li^{1

3}, Fuhua Fu^{1

3}, Jiajing Guo^{1

3}, Yang Shan^{1

3}

Affiliations

¹ Hunan Agriculture Product Processing Institute, International Joint Laboratory on Fruits and Vegetables Processing, Quality and Safety, Hunan Key Laboratory of Fruits and Vegetables Storage, Processing, Quality and Safety, Hunan Academy of Agricultural Sciences, Changsha, China.
² College of Animal Science and Technology, Hunan Agricultural University, Changsha, China.
³ Longping Branch, Graduate School of Hunan University, Changsha, China.

Abstract

Aspergillus aculeatus ZC-1005 (ZC-1005 was used as the abbreviation of this strain) is a hemicellulase-producing strain isolated from rotten citrus rind buried in the soil. Our previous study has shown its biochemical properties including high xylanase activity, mannanase activity, and degradation reaction with citrus mesocarp. In this study, we focused more on the enzyme safety evaluation and the genome sequencing via PacBio and Illumina platforms. High biological safety of the crude enzymes of ZC-1005 has been proven by the acute oral toxicity test, sub-chronic toxicity test, micronucleus test, and sperm malformation test. The genome of ZC-1005 had a GC content of 52.53%, with a size of 35,458,484 bp, and encoded 10,147 genes. Strain ZC-1005 harbored 269 glycosyl hydrolase (GH) genes of 64 families. The fungus produces cellulose-acting (GH3, GH5, GH12, and GH1) and hemicellulose-acting enzymes (GH16, GH31, GH2, and GH92). In genome annotation, we paid more attention to the genes encoding xylanase, such as gene 01512, gene 05833, gene 05469, gene 07781, gene 08432, gene 09042, gene 08008, and gene 09694. The collaboration between complete genome information and the degradation test confirmed that ZC-1005 could degrade cellulose and xylan. Our results showed that the citrus enzymatic decapsulation technology was efficacious and safe for canned citrus product processing, which may also solve the industrial waste problem. Therefore, ZC-1005 and the crude enzyme secreted from the strain were very promising to be used in the citrus processing industry.

Keywords: Aspergillus aculeatus ZC-1005; degradation; genome sequencing; safety evaluation; xylanase.