A systematic review of the proteomic profile of in vivo acquired enamel pellicle on permanent teeth

Hongying Hu; Michael Francis Burrow; Wai Keung Leung

doi:10.1016/j.jdent.2021.103799

A systematic review of the proteomic profile of in vivo acquired enamel pellicle on permanent teeth

J Dent. 2021 Oct:113:103799. doi: 10.1016/j.jdent.2021.103799. Epub 2021 Sep 4.

Authors

Hongying Hu¹, Michael Francis Burrow², Wai Keung Leung³

Affiliations

¹ Faculty of Dentistry, The University of Hong Kong, 34 Hospital Road, Sai Ying Pun, Hong Kong SAR, China. Electronic address: summerhu@connect.hku.hk.
² Faculty of Dentistry, The University of Hong Kong, 34 Hospital Road, Sai Ying Pun, Hong Kong SAR, China. Electronic address: mfburr58@hku.hk.
³ Faculty of Dentistry, The University of Hong Kong, 34 Hospital Road, Sai Ying Pun, Hong Kong SAR, China. Electronic address: ewkleung@hku.hk.

PMID: 34487803
DOI: 10.1016/j.jdent.2021.103799

Abstract

Objectives: To provide a comprehensive review on the proteomic profile of in vivo acquired enamel pellicle (AEP) formed on permanent teeth.

Data/sources: PubMed, EMBASE (Ovid) and Web of Science databases were searched for eligible studies (up to December 2020). Studies reporting mass spectrometry-based proteomic analysis of in vivo AEP were included. Risk of bias assessment was performed. Qualitative and quantitative proteomic data were extracted, integrated, then followed by bioinformatic analysis.

Study selection: Eleven studies were included, involving 122 systemically and dentally healthy adults from four different research groups. Pooled AEP samples from study participants were the normal practice for all included studies. A total of 257/93/108/870 non-redundant proteins were detectable from the in vivo ≤ 5 min/10-min/60-min/2-h AEP samples, respectively. Fifteen "core in vivo 2-h AEP proteins", generally associated with immune and/or inflammatory responses, were consistently identifiable from all four research groups. Eight included studies conducted relative quantitative proteomic analysis, while no statistical analysis could be undertaken due to the inherent limitation of the relative quantification in the proteomics analyses of these studies.

Conclusions: A systematic review on adult in vivo AEP proteomic profile was undertaken. The results provide a comprehensive appreciation of the AEP proteome in healthy individuals from in vivo sampling. Further studies are warranted to clarify the biological role of AEP on oral health, particularly at an individual level.

Clinical significance: A comprehensive appreciation of the proteomic profile of in vivo AEP in healthy individuals is essential to further understand its functions in oral health and disease. The information generated also provides insights for future studies.

Keywords: Acquired enamel pellicle; In vivo; Proteomics; Systematic review.

Publication types

Research Support, Non-U.S. Gov't
Review
Systematic Review

MeSH terms

Adult
Dental Pellicle
Humans
Mass Spectrometry
Proteome*
Proteomics*

Substances

Proteome