<b>Background and Objective:</b> <i>Moringa peregrina</i> (family Moringaceae) is a common flowering plant found in the Arabian Peninsula, Horn of Africa and Southern Sinai, Egypt. The purpose of this study was to investigate the protective activity of MP-SeNPs against BaP-induced mammal tissue injury in rats. <b>Materials and Methods:</b> MP-SeNPs were prepared and characterized in terms of particle size and zeta potential. Furthermore, the IC<sub>50</sub> of MP-SeNPs against the Mcf7 breast carcinoma cell line and LD<sub>50</sub> was evaluated. Adult albino rats weighing approximately 187±10 g was used to assess the lung protective activity of MP-SeNPs (28.7 and 71.75 mg kg<sup>1</sup> b.wt.) against BaP-induced mammal tissue injury in rats. <b>Results:</b> The mean particle size of MP-SeNPs was 134.69±8.24 nm with negative zeta potential of +26.04 with the observed shapes of nano particle was spherical. Also, IC<sub>50</sub> of MP-SeNPs against Mcf7 breast carcinoma cell line = 89.57 μg mL<sup>1</sup> and LD<sub>50</sub> equals and 1435 mg kg<sup>1</sup> b.wt., respectively. The daily oral administration of MP-SeNPs at concentrations of 28.7 and 71.75 mg kg<sup>1</sup> b.wt. for 30 days to rats treated with BaP (20 mg kg<sup>1</sup> b.wt.) resulted in a significant improvement of IL-2, IL-6 and IL-10. Oral administration of MP-SeNPs, on the other hand, increased the levels of SOD, GPx, TNF-α, iNOs and GSH as well as decreased the level of MDA in mammal tissue of BaP-treated rats. Furthermore, MP-SeNPs almost normalized these effects in mammal tissue histoarchitecture and MRI examination. <b>Conclusion:</b> The biochemical, histological and MRI findings incurrent study demonstrated that MP-SeNPs have protective activity against BaP-induced mammal tissue injury in rats.
Keywords: BaP; MP-SeNPs; Moringa peregrina; mammal tissue toxicity and cytokine storm.