Preclinical model of type 1 diabetes and myocardial ischemia/reperfusion injury in conscious rabbits-demonstration of cardioprotection with rapamycin

STAR Protoc. 2021 Aug 24;2(3):100772. doi: 10.1016/j.xpro.2021.100772. eCollection 2021 Sep 17.

Abstract

We developed a preclinical model of myocardial ischemia/reperfusion (I/R) injury in conscious diabetic rabbits to identify an early pharmacological intervention for patients with diabetes and acute myocardial infarction (AMI). Here, we describe a reproducible protocol for induction of diabetes with subsequent manifestation of myocardial I/R injury in conscious rabbits to mimic the real-life scenario observed in clinical settings. Further, we demonstrate the efficacy of rapamycin at the onset of reperfusion to limit the adverse effect of AMI. For complete details on the use and execution of this protocol, please refer to Samidurai et al. (2020).

Keywords: health sciences; metabolism; microscopy; model organisms.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Video-Audio Media

MeSH terms

  • Alloxan
  • Animals
  • Apoptosis
  • Balloon Occlusion / methods
  • Cardiotonic Agents / pharmacology*
  • Diabetes Mellitus, Experimental / physiopathology
  • Diabetes Mellitus, Type 1 / complications
  • Diabetes Mellitus, Type 1 / physiopathology*
  • Disease Models, Animal
  • Electrocardiography
  • Male
  • Myocardial Reperfusion Injury / etiology*
  • Myocardial Reperfusion Injury / pathology
  • Myocardial Reperfusion Injury / prevention & control*
  • Rabbits
  • Sirolimus / pharmacology*
  • Troponin I / blood

Substances

  • Cardiotonic Agents
  • Troponin I
  • Alloxan
  • Sirolimus