Dose-dependent efficacy of antioxidant nanoparticles on red blood cells storage

Saeid Barzegar; Mohammad Reza Rezvani; Majid Safa; Amir Amani; Alireza Abbaspour; Aliakbar Pourfathollah; Javad Hashemi; Farhad Zaker

doi:10.4103/jehp.jehp_1638_20

Dose-dependent efficacy of antioxidant nanoparticles on red blood cells storage

J Educ Health Promot. 2021 Jul 30:10:256. doi: 10.4103/jehp.jehp_1638_20. eCollection 2021.

Authors

Saeid Barzegar¹, Mohammad Reza Rezvani^{1

2

3}, Majid Safa¹, Amir Amani⁴, Alireza Abbaspour⁵, Aliakbar Pourfathollah⁶, Javad Hashemi⁵, Farhad Zaker¹

Affiliations

¹ Department of Hematology, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran.
² Pediatric Growth and Development Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences, Tehran, Iran.
³ Department of Oncology-Pathology, Immune and Gene Therapy Lab, Cancer Center Karolinska, Karolinska University Hospital Solna and Karolinska Institute, Stockholm, Sweden.
⁴ Department of Biotechnology , School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran.
⁵ Department of Pathobiology and Laboratory Sciences, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran.
⁶ Department of Immunology, School of Medicine, Iranian Blood Transfusion Research Center, Tarbiat Modares University, Tehran, Iran.

Abstract

Background: Transfusion of healthy red blood cells (RBCs) after storage is important. One of the storage lesions on blood bags is oxidative stress. One way to prevent increased oxidative stress is to use antioxidant nanoparticles (NPs). Superoxide dismutase (SOD) and catalase (CAT) play an important role in antioxidant defense on RBC. poly lactic-co-glycolic acid (PLGA) is a nontoxic biodegradable polymer that is approved by the Food and Drug Administration for drug delivery. This study aimed to assess dose-dependent efficacy of SOD-CAT-polyethylene glycol -PLGA on RBCs storage.

Materials and methods: Using a descriptive study, during 1 month, twenty donors from Bojnourd Blood Donation Center were selected. NPs with different concentrations were injected into the satellite bags after directing blood to them. On target days, experiments were performed on the samples taken. Electrospray was employed to prepare SOD-CAT-PLGA NPs. Twenty packed RBCs were isolated from the whole blood bags by the mechanical method, and certain amount of product was transferred to the satellite bags. On days 1, 7, 14, 21, 28, and 35, bags were sampled. Malondialdehyde (MDA), prooxidant-antioxidant balance (PAB), and Annexin V were performed on the samples taken. The repeated measures analysis with the help of SPSS software version 20 was performed on samples.

Results: MDA increased in both groups. The maximum increase in test group was seen in concentration 12 mg (MDA Day 14, test [1.93 ± 0.3], [P MDA < 0.001]). Maximum increase in PAB was seen in concentration 12 mg (from 444 ± 1.7 to 563 ± 2.5) (P PAB = 0.000). Furthermore, PS expression increased in the concentration of 12 mg greater than other concentration in consecutive (from 5.00 ± 0.8 to 22.26 ± 1.7, [P < 0.001]).

Conclusion: Evaluation of dose dependency showed that different concentrations of antioxidant NPs affect RBC. This effect can be changed oxidative stress and apoptosis. Using both changes to evaluate functional and toxicity can be helpful.

Keywords: Antioxidant effect; blood bank; eryptosis; nanoparticles; oxidative stress.