The role of serum macrophage migration inhibitory factor in preoperative prediction of chronic rhinosinusitis with nasal polyps endotypes

Xuan Yuan; Shaobing Xie; Qiancheng Jing; Yongchuan She; Weihong Jiang; Hua Zhang; Zhihai Xie

doi:10.1016/j.intimp.2021.108084

The role of serum macrophage migration inhibitory factor in preoperative prediction of chronic rhinosinusitis with nasal polyps endotypes

Int Immunopharmacol. 2021 Nov:100:108084. doi: 10.1016/j.intimp.2021.108084. Epub 2021 Sep 3.

Authors

Xuan Yuan¹, Shaobing Xie¹, Qiancheng Jing², Yongchuan She³, Weihong Jiang¹, Hua Zhang¹, Zhihai Xie⁴

Affiliations

¹ Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital of Central South University, Changsha, Hunan, China; Hunan Province Key Laboratory of Otolryngology Critical Diseases, Changsha, Hunan, China.
² Department of Otolaryngology Head and Neck Surgery, Changsha Central Hospital, University of South China, Changsha, Hunan, China.
³ Department of Otolaryngology Head and Neck Surgery, Changsha Hospital of Traditional Chinese Medicine, Changsha, Hunan, China.
⁴ Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital of Central South University, Changsha, Hunan, China; Hunan Province Key Laboratory of Otolryngology Critical Diseases, Changsha, Hunan, China. Electronic address: xiedoctor@csu.edu.cn.

PMID: 34482268
DOI: 10.1016/j.intimp.2021.108084

Abstract

Background: Chronic rhinosinusitis with nasal polyps (CRSwNP) is a highly heterogeneous disease and can be categorized into eosinophilic CRSwNP (eCRSwNP) and non-eosinophilic CRSwNP (neCRSwNP). Exploring effective biomarkers to distinguish endotypes is important for personalized therapies. The present study aims to evaluate the predictive value of serum MIF in CRSwNP endotypes.

Methods: One hundred and twenty CRSwNP patients, including 51 eCRSwNP and 69 neCRSwNP, 40 chronic rhinosinusitis without nasal polyps (CRSsNP) patients and 40 healthy controls (HC) were enrolled. Serum MIF levels were determined by enzyme-linked immunosorbent assay (ELISA). The patients' clinical variables were analyzed for correlations with serum MIF. Receiver operating characteristic (ROC) curve and multivariate analysis were utilized to assess the predictive value of serum MIF in CRSwNP endotypes.

Results: The serum MIF levels were significantly higher in CRSwNP group than CRSsNP group and HC group (P < 0.001), and the serum MIF levels were increased in eCRSwNP compared to neCRSwNP group (P = 0.006). Elevated serum MIF levels were significantly correlated with blood eosinophil (B-EOS) count (r = 0.411, P < 0.001), B-EOS percentage (r = 0.377, P < 0.001), visual analog scale score (r = 0.204, P = 0.025), tissue eosinophil (T-EOS) count (r = 0.705, P < 0.001) and T-EOS percentage (r = 0.671, P < 0.001) in CRSwNP patients. ROC curve demonstrated that serum MIF exhibited good preoperative prediction in CRSwNP endotypes (area under the curve = 0.925, P < 0.001). Multivariate regression analysis showed that serum MIF was an independent factor associated with CRSwNP endotypes.

Conclusions: This was the first study identifying serum MIF as a possible specific biomarker for preoperatively distinguishing CRSwNP endotypes. Furthermore, the serum MIF levels were found to be closely associated with the degree of mucosal eosinophil infiltration.

Keywords: Biomarker; Chronic rhinosinusitis with nasal polyps; Endotypes; Eosinophil; Macrophage migration inhibitory factor.

MeSH terms

Adult
Biomarkers / blood
Case-Control Studies
Chemotaxis, Leukocyte
Chronic Disease
Enzyme-Linked Immunosorbent Assay*
Eosinophils / immunology
Eosinophils / metabolism
Female
Humans
Intramolecular Oxidoreductases / blood*
Macrophage Migration-Inhibitory Factors / blood*
Male
Middle Aged
Nasal Mucosa / immunology
Nasal Mucosa / metabolism
Nasal Polyps / blood*
Nasal Polyps / diagnosis
Nasal Polyps / immunology
Predictive Value of Tests
Rhinitis / blood*
Rhinitis / diagnosis
Rhinitis / immunology
Sinusitis / blood*
Sinusitis / diagnosis
Sinusitis / immunology
Up-Regulation
Young Adult

Substances

Biomarkers
Macrophage Migration-Inhibitory Factors
Intramolecular Oxidoreductases
MIF protein, human