Characterisation of blaNDM-5 and blaKPC-2 co-occurrence in K64-ST11 carbapenem-resistant Klebsiella pneumoniae

Renjing Hu; Qin Li; Feiyang Zhang; Manlin Ding; Jun Liu; Yingshun Zhou

doi:10.1016/j.jgar.2021.08.009

Characterisation of bla_NDM-5 and bla_KPC-2 co-occurrence in K64-ST11 carbapenem-resistant Klebsiella pneumoniae

J Glob Antimicrob Resist. 2021 Dec:27:63-66. doi: 10.1016/j.jgar.2021.08.009. Epub 2021 Sep 2.

Authors

Renjing Hu¹, Qin Li², Feiyang Zhang², Manlin Ding², Jun Liu³, Yingshun Zhou⁴

Affiliations

¹ Department of Laboratory Medicine, the Affiliated Wuxi No. 2 People's Hospital of Nanjing Medical University, Wuxi, China.
² Department of Pathogenic Biology, School of Basic Medicine, Southwest Medical University, Luzhou, Sichuan, China.
³ Department of Laboratory Medicine, the Fifth People's Hospital of Wuxi, affiliated to Jiangnan University, Wuxi, Jiangsu, China. Electronic address: 15949255828@126.com.
⁴ Department of Pathogenic Biology, School of Basic Medicine, Southwest Medical University, Luzhou, Sichuan, China. Electronic address: yingshunzhou@swmu.edu.cn.

PMID: 34482020
DOI: 10.1016/j.jgar.2021.08.009

Abstract

Objectives: The aim of this study was to characterise the co-occurrence of bla_KPC and bla_NDM in a K64-ST11 carbapenem-resistant Klebsiella pneumoniae strain.

Methods: Antimicrobial susceptibility was determined by the disk diffusion method. Whole-genome sequencing was performed using Illumina MiSeq and PacBio II sequencers. High-quality reads were de novo assembled using the SOAPdenovo package. Genome annotation was performed using the NCBI Prokaryotic Genome Annotation Pipeline (PGAP), and genome characteristics were analysed using bioinformatics methods.

Results: Klebsiella pneumoniae strain KPWX136 was resistant to most of the tested antibiotics, being susceptible only to polymyxin B and tigecycline. The genome of strain KPWX136 is composed of a single chromosome (5 473 976 bp) and six plasmids including pA (191 359 bp), pB (134 972 bp), pC (117 844 bp), pD (87 095 bp), pE (11 970 bp) and pF (5596 bp). Complete sequence analysis revealed the resistome of isolate KPWX136, which included bla_KPC-2 and bla_NDM-5 together with 23 other resistance genes, of which 6 resistance genes were located on the chromosome and 19 on plasmids. Virulome analysis showed that KPWX136 carried a large number of virulence-associated genes. Meanwhile, 26 genomic islands and 6 prophages were predicted within the genome.

Conclusion: Genetic characterisation of K. pneumoniae KPWX136 co-harbouring bla_NDM-5 and bla_KPC-2 showed that it carried not only 25 resistance genes and a large number virulence factors but also various mobile genetic elements (MGEs) such as plasmids and genomic islands. Therefore, we must be alert to the transmission of resistance genes and virulence determinants via MGEs.

Keywords: Carbapenemase-producing Enterobacteriaceae; Klebsiella pneumoniae; bla(KPC-2); bla(NDM-5).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbapenems / pharmacology
Humans
Klebsiella Infections* / epidemiology
Klebsiella pneumoniae* / genetics
Microbial Sensitivity Tests
Multilocus Sequence Typing

Substances

Carbapenems