MicroRNA ssc-miR-124a exhibits antiviral activity against porcine reproductive and respiratory syndrome virus via suppression of host genes CD163

Na Li; Ke Huang; Yujing Chen; Zhengyang Huang; Yiyi Zhang; ChaoLiang Leng; Yangkun Liu; Jianzhou Shi; Shuqi Xiao; Lunguang Yao

doi:10.1016/j.vetmic.2021.109216

MicroRNA ssc-miR-124a exhibits antiviral activity against porcine reproductive and respiratory syndrome virus via suppression of host genes CD163

Vet Microbiol. 2021 Oct:261:109216. doi: 10.1016/j.vetmic.2021.109216. Epub 2021 Aug 24.

Authors

Affiliations

¹ Henan Provincal Engineering and Technology Center of Health Products for Livestock and Poultry, Nanyang, 473061, China; Key Laboratory of Ecological Security and Collaborative Innovation Centre of Water Security for Water Source Region of Mid-Line of South-to-North Diversion Project of Henan Province, Nanyang, 473061, China; School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang, 473061, China.
² School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang, 473061, China.
³ College of Veterinary Medicine, Northwest A&F University, No. 22 Xinong Road, Yangling, 712100, Shaanxi, China. Electronic address: xiaoshuqi@nwsuaf.edu.cn.
⁴ Henan Provincal Engineering and Technology Center of Health Products for Livestock and Poultry, Nanyang, 473061, China; Key Laboratory of Ecological Security and Collaborative Innovation Centre of Water Security for Water Source Region of Mid-Line of South-to-North Diversion Project of Henan Province, Nanyang, 473061, China; School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang, 473061, China. Electronic address: lunguangyao@163.com.

PMID: 34481271
DOI: 10.1016/j.vetmic.2021.109216

Abstract

Porcine reproductive and respiratory syndrome (PRRS) is a serious infectious disease in the swine industry, which causes severe economic losses to current swine production worldwide. There are no effective antiviral strategies for preventing this disease. Previous studies showed that microRNAs (miRNAs) play important role in virus-host interactions. In this study, we demonstrated that the expression level of ssc-miR-124a was significantly downregulated during both high and low pathogenic PRRSV infection. Overexpression of ssc-miR-124a markedly inhibits PRRSV replication in PAMs. Luciferase reporter experiments and RISC immunoprecipitation assay were used to identify the ssc-miR-124a could directly target the 3'UTR of pig CD163 mRNA in a sequence-specific manner and that CD163 mRNA and protein levels were reduced in PAMs overexpressing ssc-miR-124a. These data not only provide new insights into virus-host interactions during PRRSV infection, but also suggest potential new antiviral strategies against PRRSV infection in the future.

Keywords: CD163; PRRSV; microRNA; ssc-miR-124a.

MeSH terms

Animals
Antigens, CD / genetics*
Antigens, Differentiation, Myelomonocytic / genetics*
Gene Expression Regulation / immunology*
Host Microbial Interactions / genetics*
Porcine Reproductive and Respiratory Syndrome / immunology*
Porcine Reproductive and Respiratory Syndrome / virology*
Porcine respiratory and reproductive syndrome virus / immunology
Receptors, Cell Surface / genetics*
Swine

Substances

Antigens, CD
Antigens, Differentiation, Myelomonocytic
CD163 antigen
Receptors, Cell Surface