Abstract
Amanitin is used extensively as a research tool to inhibit RNA Pol II thereby implicating its role in mRNA transcription. Recently, amanitin has gained traction as a toxic payload for targeted therapy. Here we report the first-ever photocaged amanitin analog, that is non-toxic and can be pre-loaded into cells. Light provides a means to inhibit RNA Pol II and provoke cell death on-demand.
MeSH terms
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Amanitins / chemical synthesis
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Amanitins / chemistry
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Amanitins / pharmacology*
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Animals
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CHO Cells
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Cell Death / drug effects
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Cell Survival / drug effects
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Cricetulus
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Dose-Response Relationship, Drug
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Molecular Structure
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Photochemical Processes
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Prodrugs / chemical synthesis
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Prodrugs / chemistry
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Prodrugs / pharmacology*
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RNA Polymerase II / antagonists & inhibitors*
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RNA Polymerase II / metabolism
Substances
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Amanitins
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Prodrugs
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amatoxin
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RNA Polymerase II