Controlling cell elongation and orientation by using microstructural nanofibre scaffolds for accelerating tissue regeneration

Abstract

The topographic surface conditions of scaffolds can regulate cellular behaviours, such as by stimulating cellular migration and morphological changes to wound sites and have the potential to promote tissue regeneration. In this research, four types of engineered topographic surfaces, including arrays of hemisphere, pyramid, semi-cylinder, and triangle prism microstructures, were patterned on silicon moulds using microfabrication processes. The microstructural patterns were transferred onto the surface of polycaprolactone membranes and nanofibrous scaffolds by combining with the moulding approach and electrospinning technique, respectively. In vitro experimental results demonstrated that the triangular microstructural nanofibre provided a strong guiding performance to the filopodia of cultured C2C12 myoblast cells, thus inducing cellular elongation and alignment in the longitudinal direction and forming an elongated cell morphology. The cultured cells rapidly transitioned into an elongated morphology at an aspect ratio of 17.33 after 24 h of incubation, with 70% of the cell elongates aligning with the direction of triangular microstructural patterns. The cells cultured on the triangular microstructural nanofibre elongated four-fold compared with those in the flat nanofibre scaffold. Moreover, an in vivo study showed that wounds treated with the triangular microstructural nanofibre scaffold achieved 95.04% wound closure after 14 days and completed the reepithelialisation with an ordered collagen arrangement. Therefore, we believe that the engineered triangular nanofibrous scaffold may accelerate tissue regeneration and has potential for wound healing applications.

Keywords: Cell behaviour; Microstructural nanofibre; Surface topography; Tissue regeneration; Wound healing.