Method of Using Raman Spectroscopy to Understand the Conformation of Fibrous Proteins

Wenli Gao; Ting Shu; Qiang Liu; Hongchong Guo; Shengjie Ling; Liang Zhou

doi:10.1007/978-1-0716-1574-4_20

Method of Using Raman Spectroscopy to Understand the Conformation of Fibrous Proteins

Methods Mol Biol. 2021:2347:229-239. doi: 10.1007/978-1-0716-1574-4_20.

Authors

Wenli Gao¹, Ting Shu², Qiang Liu², Hongchong Guo², Shengjie Ling², Liang Zhou³

Affiliations

¹ Key Lab of State Forest and Grassland Administration on Wood Quality Improvement & High Efficient Utilization, School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei, Anhui, China.
² School of Physical Science and Technology, ShanghaiTech University, Shanghai, China.
³ Key Lab of State Forest and Grassland Administration on Wood Quality Improvement & High Efficient Utilization, School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei, Anhui, China. mcyjs1@ahau.edu.cn.

PMID: 34472069
DOI: 10.1007/978-1-0716-1574-4_20

Abstract

Raman spectroscopy has been widely used in the research of fibrous proteins because of the insensitivity to moisture, less amount of sample, and better signal-to-noise ratio. In recent years, Raman spectroscopy is adopted to investigate the secondary structures of solid or aqueous protein, the conformation transition under different conditions (concentration, temperature, pressure, pH, chemical modification, external force, etc.), the orientation of the molecular chains, and some important chemical bonds. Here, we will introduce the methods for using Raman spectroscopy to analyze the conformation and orientation of samples, which would be an efficient method to get the "structure-property" relationship.

Keywords: Conformation; Fibrous Proteins; Raman Spectroscopy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Protein Conformation
Protein Structure, Secondary
Scleroproteins
Spectrum Analysis, Raman*
Temperature

Substances

Scleroproteins