Conductive materials amendment in anaerobic digestion (AD) is a promising strategy for boosting the methanogenesis process. Despite mixing is a critical parameter, the behavior of digesters amended with conductive additives upon different mixing conditions has rarely been investigated. This study investigated continuous mixing, intermittent mixing (10 min in every 12 h), and non-mixing conditions for digesters amended with granular activated carbon (GAC) and powdered activated carbon (PAC). The non-mixed GAC digester provided the highest methane yield (318 ± 28 mL/g COD) from synthetic blackwater, while intermittently mixed GAC and control exhibited similar methane yields (290-294 mL/g COD). For non-mixed systems, microbial richness and diversity increased with GAC and PAC amendment. In contrast, continuous and intermittent mixing increased microbial diversity and richness in control reactors while reduced the same in GAC and PAC amended reactors. Overall, various mixing conditions distinctly changed the degree of enrichment/retention of microbes and consequently influenced methane recovery.
Keywords: Anaerobic digestion; Biomethane; Blackwater; Conductive materials; Mixing conditions.
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