Physical and chemical treatments of Tungsten smelting wastewater, with high salt content and low C/N ratio, are often tedious. As a solution, this study suggested a simultaneous nitrification and denitrification membrane bioreactor (SND-MBR) for salinity gradient domestication. During the salinity acclimation period, we observed 20% and 11% removal of NH4+-N and Chemical Oxygen Demand (COD), respectively. However, the SND efficiency reached 95.55% after stable operation at 3.0% salinity. Through stoichiometric and kinetic analyses, we confirmed that increased salinity significantly inhibited electron transport system activity, nitrification, and denitrification, evidenced by the extremely low ammonia monooxygenase and nitrite reductase activities. Further high-throughput sequencing showed that Nitrosomonas dominated the functional microbial flora succession and denitrification in high salinity environments. In comparison with a control, the Kyoto Encyclopedia of Genes and Genomes analysis showed that wastewater salinity weakened the functional gene level of MBR microbial flora, and the enzyme key to the assimilation nitrate reduction changed from nitrate reductase to assimilation nitrate reductase.
Keywords: Membrane bioreactor (MBR); Microbial community; Nitrification activity; Salinity; Simultaneous nitrification and denitrification (SND).
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