Expression and clinical significance of UBE2V1 in cervical cancer

Zhishuai Ren; Zhendong Liu; Shenqian Ma; Junming Yue; Jinming Yang; Ruiya Wang; Yanzheng Gao; Yuqi Guo

doi:10.1016/j.bbrep.2021.101108

Expression and clinical significance of UBE2V1 in cervical cancer

Biochem Biophys Rep. 2021 Aug 18:28:101108. doi: 10.1016/j.bbrep.2021.101108. eCollection 2021 Dec.

Authors

Zhishuai Ren¹, Zhendong Liu², Shenqian Ma¹, Junming Yue³, Jinming Yang⁴, Ruiya Wang¹, Yanzheng Gao², Yuqi Guo^{5

6}

Affiliations

¹ People's Hospital of Zhengzhou University, Henan Provincial People's Hospital, Zhengzhou, 450003, Henan, China.
² Department of Surgery of Spine and Spinal Cord, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University, Zhengzhou, 450003, Henan, China.
³ Department of Pathology, The University of Tennessee Health Science Center, 19 S. Manassas St., Rm. 266, Memphis, TN, 38163, USA.
⁴ Department of Pharmacology, Vanderbilt University Medical Center, Nashville, TN, 37240, USA.
⁵ Department of Obstetrics and Gynecology, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University, Zhengzhou, 450003, Henan, China.
⁶ Henan International Joint Laboratory for Gynecological Oncology and Nanomedicine, Zhengzhou, 450003, Henan, China.

Abstract

The majority of cervical cancer (CC) patients are caused by the high-risk human papillomavirus (HPV) infection Although they are preventable and controllable, the mortality rate is still high. It is essential to identify the biomarkers for early screening and diagnosis of CC to improve the prognosis of patients with CC. The conjugating enzyme 2 (E2) family members are the key components of ubiquitin protease system. However, the role of E2 family in CC remains unclear. We aimed to investigate the role of UBE2V1, a ubiquitin binding E2 enzyme variant protein (ube2v) without conserved cysteine residues required for E2s catalytic activity in CC. In this study, we first studied the expression of UBE2V1 in CC by real time quantitative PCR (RT-qPCR), and then, the clinical information of 191 CC patients in TCGA database was retrieved to explore the relationship between the expression of UBE2V1 and the occurrence and development of CC by examining the translational profile and methylation, the high expression of UBE2V1 was well correlated to the poor prognosis of patients, indicating that UBE2V1 is an independent risk factor for the prognosis of CC patients. The expression of UBE2V1 was also correlated with clinical stages, tumor grades and TNM stages of CC. In addition, the expression of UBE2V1 was slightly negatively correlated with the methylation at the multiple methylation sites. our study revealed the relationship between UBE2V1 and the occurrence and development of CC from the level of transcriptional profile and DNA methylation. UBE2V1 is a novel candidate biomarker for the diagnosis, screening and prognosis of CC.

Keywords: Biomarker; Cervical cancer; Oncology; UBE2V1.