Pepsin pretreatment corrects underestimation of 25-hydroxyvitamin D measurement by an automated immunoassay in subjects with high vitamin D binding protein levels

Clin Chem Lab Med. 2021 Sep 1;60(1):e18-e20. doi: 10.1515/cclm-2021-0722. Print 2022 Jan 26.

Authors

Anneke C Muller Kobold¹, Ido P Kema¹, Janneke Dijck-Brouwer¹, Karin Koerts¹, Michiel Weening¹, Twan Storteboom¹, Jenny E Kootstra-Ros¹, Martijn van Faassen¹, Ineke J Riphagen¹

Affiliation

¹ Department of Laboratory Medicine, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.

PMID: 34464525
DOI: 10.1515/cclm-2021-0722

No abstract available

Keywords: immunoassay; liquid chromatography-tandem mass spectrometry (LC-MS/MS); pepsin digestion; vitamin D; vitamin D-binding protein (VDBP).

Publication types

Letter

MeSH terms

25-Hydroxyvitamin D 2
Humans
Immunoassay
Pepsin A* / metabolism
Vitamin D / analogs & derivatives
Vitamin D / metabolism
Vitamin D-Binding Protein*

Substances

Vitamin D-Binding Protein
Vitamin D
25-Hydroxyvitamin D 2
25-hydroxyvitamin D
Pepsin A