Scaffold-guided bone regeneration in large volume tibial segmental defects

Jan Henkel; Flavia Medeiros Savi; Arne Berner; Stephanie Fountain; Siamak Saifzadeh; Roland Steck; Devakar R Epari; Maria A Woodruff; Mark Knackstedt; Michael A Schuetz; Dietmar W Hutmacher

doi:10.1016/j.bone.2021.116163

Scaffold-guided bone regeneration in large volume tibial segmental defects

Bone. 2021 Dec:153:116163. doi: 10.1016/j.bone.2021.116163. Epub 2021 Aug 28.

Authors

Affiliations

¹ Centre in Transformative Biomimetics in Bioengineering, Queensland University of Technology, Kelvin Grove, Australia; Department of Trauma Surgery, Lutheran Hospital Goettingen-Weende, Goettingen, Germany.
² Centre in Transformative Biomimetics in Bioengineering, Queensland University of Technology, Kelvin Grove, Australia; ARC Training Centre for Multiscale 3D Imaging, Modelling and Manufacturing, Australia; Faculty of Engineering, School of Mechanical Medical & Process Engineering, Queensland University of Technology, Kelvin Grove, Australia.
³ Department of Trauma Surgery, University Hospital of Regensburg, Regensburg, Germany; Australian Research Council Industrial Transformation Training Centre in Additive Biomanufacturing, Australia.
⁴ Faculty of Engineering, School of Mechanical Medical & Process Engineering, Queensland University of Technology, Kelvin Grove, Australia.
⁵ Centre in Transformative Biomimetics in Bioengineering, Queensland University of Technology, Kelvin Grove, Australia; Faculty of Engineering, School of Mechanical Medical & Process Engineering, Queensland University of Technology, Kelvin Grove, Australia; Medical Engineering Research Facility, Queensland University of Technology, Brisbane, Australia.
⁶ Medical Engineering Research Facility, Queensland University of Technology, Brisbane, Australia.
⁷ Department of Applied Mathematics, Australian National University (ANU), Canberra, Australia.
⁸ Jamieson Trauma Institute, Royal Brisbane Hospital, Herston, Australia.
⁹ Centre in Transformative Biomimetics in Bioengineering, Queensland University of Technology, Kelvin Grove, Australia; ARC Training Centre for Multiscale 3D Imaging, Modelling and Manufacturing, Australia; Faculty of Engineering, School of Mechanical Medical & Process Engineering, Queensland University of Technology, Kelvin Grove, Australia; Australian Research Council Industrial Transformation Training Centre in Additive Biomanufacturing, Australia; Jamieson Trauma Institute, Royal Brisbane Hospital, Herston, Australia. Electronic address: dietmar.hutmacher@qut.edu.au.

PMID: 34461285
DOI: 10.1016/j.bone.2021.116163

Abstract

Large volume losses in weight bearing long bones are a major challenge in clinical practice. Despite multiple innovations over the last decades, significant limitations subsist in current clinical treatment options which is driving a strong clinical demand for clinically translatable treatment alternatives, including bone tissue engineering applications. Despite these shortcomings, preclinical large animal models of large volume segmental bone defects to investigate the regenerative capacity of bone tissue engineering strategies under clinically relevant conditions are rarely described in literature. We herein present a newly established preclinical ovine animal model for the treatment of XL volume (19 cm³) segmental tibial defects. In eight aged male Merino sheep (age > 6 years) a mid-diaphyseal tibial segmental defect was created and stabilized with a 5.6 mm Dynamic Compression Plate (DCP). We present short-term (3 months) and long-term (12-15 months) results of a pilot study using medical grade Polycaprolactone-Tricalciumphosphate (mPCL-TCP) scaffolds combined with a dose of 2 mg rhBMP-7 delivered in Platelet-Rich- Plasma (PRP). Furthermore, detailed analyses of the mechanical properties of the scaffolds as well as interfragmentary movement (IFM) and DCP-surface strain in vitro and a comprehensive description of the surgical and post-surgery protocol and post-mortem analysis is given.

Keywords: Bone morphogenetic protein; Bone tissue engineering; Large volume bone defect; Ovine; Polycaprolactone; Preclinical animal model; Scaffold; Sheep.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bone Regeneration*
Bone and Bones
Male
Pilot Projects
Sheep
Tibia / diagnostic imaging
Tibia / surgery
Tissue Engineering*
Tissue Scaffolds