Paeoniflorin inhibits proliferation and migration of psoriatic keratinocytes via the lncRNA NEAT1/miR-3194-5p/Galectin-7 axis

Anticancer Drugs. 2022 Jan 1;33(1):e423-e433. doi: 10.1097/CAD.0000000000001225.

Abstract

To investigate the mechanism underlying the effect of paeoniflorin (PF) on the proliferation and migration of psoriatic keratinocytes. The expressions of long noncoding RNA NEAT1, miR-3194-5p and Galectin-7 in skin tissues from psoriatic patients and healthy controls were detected. Psoriatic HaCat cells were used to investigate the function of NEAT1 and Galectin-7 as well as the effect and mechanism of PF in psoriasis. MTT, colony formation and scratch assays were used to assess the proliferation and migration of psoriatic HaCat cells. Dual-luciferase reporter assay was used to validate the interactions among NEAT1, miR-3194-5p and Galectin-7. NEAT1 and Galectin-7 were lowly expressed and miR-3194-5p was highly expressed in psoriatic patients. PF suppressed the proliferation and migration of psoriatic HaCat cells by elevating the expressions of NEAT1 and Galectin-7. NEAT1 positively mediated the expression of Galectin-7 by targeting miR-3194-5p. PF controls the proliferation and migration of psoriatic HaCat cells via the NEAT1/miR-3194-5p/Galectin-7 axis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / drug effects
  • Cell Movement / drug effects
  • Cell Proliferation / drug effects
  • Galectins / drug effects*
  • Glucosides / pharmacology*
  • HEK293 Cells
  • HaCaT Cells
  • Humans
  • Keratinocytes / drug effects*
  • MicroRNAs / drug effects*
  • Monoterpenes / pharmacology*
  • Psoriasis / pathology
  • RNA, Long Noncoding / drug effects*
  • Signal Transduction
  • Up-Regulation

Substances

  • Galectins
  • Glucosides
  • LGALS7 protein, human
  • MIRN3194 microRNA, human
  • MicroRNAs
  • Monoterpenes
  • NEAT1 long non-coding RNA, human
  • RNA, Long Noncoding
  • peoniflorin