Differential phenotype and behavior in culture of CD34 positive cells from peripheral blood and adipose tissue

Harald Froehlich; Robert D Simari; Barry A Boilson

doi:10.1016/j.heliyon.2021.e07779

Differential phenotype and behavior in culture of CD34 positive cells from peripheral blood and adipose tissue

Heliyon. 2021 Aug 12;7(8):e07779. doi: 10.1016/j.heliyon.2021.e07779. eCollection 2021 Aug.

Authors

Harald Froehlich^{1

2}, Robert D Simari³, Barry A Boilson¹

Affiliations

¹ Department of Cardiovascular Diseases, Mayo Clinic, Rochester, Minnesota, USA.
² Department of Internal Medicine and Emergency Medicine, LKH Graz II, Graz, Austria.
³ Department of Cardiovascular Medicine, University of Kansas School of Medicine, USA.

Abstract

The localization and quantification of endothelial progenitor cells (EPCs) are controversial. Circulating CD34 + cells in blood have been identified as EPCs and as biomarkers of cardiovascular disease. We discuss in this paper the current data describing differential phenotype and behavior in vitro of CD34 positive cells from the circulation and adipose tissue (AT). We also describe in brief our own findings from CD34 + cells isolated from leukopheresis cones derived from healthy platelet donors and from patients undergoing bariatric surgery. We conclude that CD34 + cells in blood and in AT are different in antigenic profile and behavior in culture. The findings described assert that CD34 + cells detected in blood previously identified as biomarkers of cardiovascular disease are predominantly HPCs rather than EPCs, and that true CD34 + EPCs can be readily identified and extracted from AT, supportive of the current evidence which suggests EPCs are resident in the tissue vasculature.

Keywords: Adipose tissue; Cell culture; Endothelial progenitor cells; Flow cytometry; Hematopoietic progenitor cells; Peripheral blood.

Publication types

Review