Dataset for laboratory treatability experiment with activated carbon and bioamendments to enhance biodegradation of chlorinated ethenes

Cecilie B Ottosen; Melissa Skou; Emilie Sammali; Jeremy Zimmermann; Daniel Hunkeler; Poul L Bjerg; Mette M Broholm

doi:10.1016/j.dib.2021.107291

Dataset for laboratory treatability experiment with activated carbon and bioamendments to enhance biodegradation of chlorinated ethenes

Data Brief. 2021 Aug 14:38:107291. doi: 10.1016/j.dib.2021.107291. eCollection 2021 Oct.

Authors

Cecilie B Ottosen¹, Melissa Skou¹, Emilie Sammali², Jeremy Zimmermann², Daniel Hunkeler², Poul L Bjerg¹, Mette M Broholm¹

Affiliations

¹ Department of Environmental Engineering, Technical University of Denmark, Bygningstorvet, building 115, 2800 Kgs., Lyngby, Denmark.
² Center for Hydrogeology and Geothermics (CHYN), University of Neuchâtel, Rue Emile Argand 11, CH-2000, Neuchâtel, Switzerland.

Abstract

This dataset describes the outcome of a laboratory trichloroethene (TCE) treatability experiment with liquid activated carbon and bioamendments. The treatability experiment included unamended microcosms, bioamended microcosms with a Dehalococcoides containing culture and electron donor, and bioamended microcosms including liquid activated carbon (PlumeStop®). Data were collected frequently over an 85-day experimental period. Data were collected for the following parameters: redox sensitive species, chlorinated ethenes, non-chlorinated end-products, electron donors, compound specific isotopes, specific bacteria and functional genes. The reductive dechlorination of TCE could be described by a carbon isotope enrichment factor (ε_C) of -7.1 ‰. In the amended systems, the degradation rates for the TCE degradation were 0.08-0.13 d^-1 and 0.05-0.09 d^-1 determined by concentrations and isotope fractionation, respectively. Dechlorination of cis-DCE was limited. This dataset assisted in identifying the impact of different bioamendments and activated carbon on biodegradation of chlorinated ethenes. The dataset is useful in optimising design and setup for future laboratory and field investigations. This study provides novel information on the effect of low dose liquid activated carbon on chlorinated ethenes degradation by applying isotopic and microbial techniques, and by linking the outcome to a field case study. The data presented in this article are related to the research article "Assessment of chlorinated ethenes degradation after field scale injection of activated carbon and bioamendments: Application of isotopic and microbial analyses" (Ottosen et al., 2021).

Keywords: Compound specific isotope analysis; Liquid activated carbon; Molecular biology; Multiple Lines of Evidence; Reductive dechlorination.