Pseudomonas syringae Flood-inoculation Method in Arabidopsis

Yasuhiro Ishiga; Takako Ishiga; Yuki Ichinose; Kirankumar S Mysore

doi:10.21769/BioProtoc.2106

Pseudomonas syringae Flood-inoculation Method in Arabidopsis

Bio Protoc. 2017 Jan 20;7(2):e2106. doi: 10.21769/BioProtoc.2106.

Authors

Yasuhiro Ishiga¹, Takako Ishiga¹, Yuki Ichinose², Kirankumar S Mysore³

Affiliations

¹ Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan.
² Laboratory of Plant Pathology and Genetic Engineering, Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan.
³ Plant Biology Division, the Samuel Roberts Noble Foundation, Ardmore, USA.

Abstract

Pseudomonas syringae pv. tomato strain DC3000 (Pto DC3000), which causes bacterial speck disease of tomato, has been used as a model pathogen because of its pathogenicity on Arabidopsis thaliana. Here, we demonstrate a rapid and reliable flood-inoculation method based on young Arabidopsis seedlings grown on one-half strength MS medium. We also describe a method to evaluate the bacterial growth in Arabidopsis.

Keywords: Arabidopsis thaliana; Pathogenicity; Plant innate immunity; Pseudomonas syringae.