Examination of the Interaction between a Membrane Active Peptideand Artificial Bilayers by Dual Polarisation Interferometry

Jennifer A E Payne; Tzong HsienLee; Marilyn A Anderson; Marie-Isabel Aguilar

doi:10.21769/BioProtoc.2087

Examination of the Interaction between a Membrane Active Peptideand Artificial Bilayers by Dual Polarisation Interferometry

Bio Protoc. 2017 Jan 5;7(1):e2087. doi: 10.21769/BioProtoc.2087.

Authors

Jennifer A E Payne^{1

2}, Tzong HsienLee², Marilyn A Anderson¹, Marie-Isabel Aguilar²

Affiliations

¹ Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria, Australia.
² Monash Biomedicine Discovery Institute and Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia.

Abstract

Examining the interaction of peptides with lipid bilayers to determine binding kinetics is often performed using surface plasmon resonance (SPR). Here we describe the technique of dual polarisation interferometry (DPI) that provides not only information on the kinetics of the peptide binding to the bilayer, but also how the peptide affects the lipid order of the bilayer.

Keywords: Bilayer; Kinetics; Lipid order; Membrane interaction; Peptide; Protein-lipid interaction.