Optical coherence microangiography of the mouse kidney for diagnosis of circulatory disorders

Arkady S Abdurashitov; Ekaterina S Prikhozhdenko; Oksana A Mayorova; Valentina O Plastun; Olga I Gusliakova; Natalia A Shushunova; Oleg A Kulikov; Valery V Tuchin; Gleb B Sukhorukov; Olga A Sindeeva

doi:10.1364/BOE.430393

Optical coherence microangiography of the mouse kidney for diagnosis of circulatory disorders

Biomed Opt Express. 2021 Jun 28;12(7):4467-4477. doi: 10.1364/BOE.430393. eCollection 2021 Jul 1.

Authors

Arkady S Abdurashitov^{1

2}, Ekaterina S Prikhozhdenko³, Oksana A Mayorova³, Valentina O Plastun³, Olga I Gusliakova³, Natalia A Shushunova³, Oleg A Kulikov⁴, Valery V Tuchin^{3

5

6}, Gleb B Sukhorukov^{1

7}, Olga A Sindeeva^{1

3

8}

Affiliations

¹ Skolkovo Institute of Science and Technology, Skolkovo Innovation Center, 3 Nobel str., Moscow 143005, Russia.
² a.abdurashitov@skoltech.ru.
³ Science Medical Center, Saratov State University, 83 Astrakhanskaya str., Saratov 410012, Russia.
⁴ Ogarev Mordovia State University, 68 Bolshevistskaya str., Saransk 430005, Russia.
⁵ Interdisciplinary Laboratory of Biophotonics, National Research Tomsk State University, 36 Lenina Avenue, Tomsk 634050, Russia.
⁶ Laboratory of Laser Diagnostics of Technical and Living Systems, Institute of Precision Mechanics and Control of the Russian Academy of Science, 24 Rabochaya Str., Saratov 410028, Russia.
⁷ School of Engineering and Materials Science, Queen Mary University of London, Mile End, Eng, 215, London E1 4NS, United Kingdom.
⁸ o.sindeeva@skoltech.ru.

Abstract

Optical coherence tomography (OCT) has become widespread in clinical applications in which precise three-dimensional functional imaging of living organs is required. Nevertheless, the kidney is inaccessible for the high resolution OCT imaging due to a high light attenuation coefficient of skin and soft tissues that significantly limits the penetration depth of the probing laser beam. Here, we introduce a surgical protocol and fixation scheme that enables functional visualization of kidney's peritubular capillaries via OCT microangiography. The model of reversible/irreversible glomerulus embolization using drug microcarriers confirms the ability of OCT to detect circulatory disorders. This approach can be used for choosing optimal carriers, their dosages and diagnosis of other blood flow pathologies.