Evidence That the Etiology of Congenital Hypopituitarism Has a Major Genetic Component but Is Infrequently Monogenic

Youn Hee Jee; Mariam Gangat; Olga Yeliosof; Adrian G Temnycky; Selena Vanapruks; Philip Whalen; Evgenia Gourgari; Cortney Bleach; Christine H Yu; Ian Marshall; Jack A Yanovski; Kathleen Link; Svetlana Ten; Jeffrey Baron; Sally Radovick

doi:10.3389/fgene.2021.697549

Evidence That the Etiology of Congenital Hypopituitarism Has a Major Genetic Component but Is Infrequently Monogenic

Front Genet. 2021 Aug 11:12:697549. doi: 10.3389/fgene.2021.697549. eCollection 2021.

Authors

Affiliations

¹ Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, United States.
² Division of Pediatric Endocrinology Rutgers Robert Wood Johnson Medical School Child Health Institute of New Jersey, New Brunswick, NJ, United States.
³ Division of Pediatric Endocrinology, MedStar Georgetown University Hospital, Washington, DC, United States.
⁴ Division of Pediatric Endocrinology, Walter Reed National Military Medical Center, Bethesda, MD, United States.
⁵ Section of Adult and Pediatric Endocrinology and Metabolism, University of Chicago, Chicago, IL, United States.
⁶ Division of Pediatric Endocrinology, Pediatric Subspecialists of Virginia, Fairfax, VA, United States.
⁷ Pediatric Endocrinology, Richmond University Medical Center, Staten Island, NY, United States.

Abstract

Purpose: Congenital hypopituitarism usually occurs sporadically. In most patients, the etiology remains unknown.

Methods: We studied 13 children with sporadic congenital hypopituitarism. Children with non-endocrine, non-familial idiopathic short stature (NFSS) (n = 19) served as a control group. Exome sequencing was performed in probands and both unaffected parents. A burden testing approach was used to compare the number of candidate variants in the two groups.

Results: First, we assessed the frequency of rare, predicted-pathogenic variants in 42 genes previously reported to be associated with pituitary gland development. The average number of variants per individual was greater in probands with congenital hypopituitarism than those with NFSS (1.1 vs. 0.21, mean variants/proband, P = 0.03). The number of probands with at least 1 variant in a pituitary-associated gene was greater in congenital hypopituitarism than in NFSS (62% vs. 21%, P = 0.03). Second, we assessed the frequency of rare, predicted-pathogenic variants in the exome (to capture undiscovered causes) that were inherited in a fashion that could explain the sporadic occurrence of the proband's condition with a monogenic etiology (de novo mutation, autosomal recessive, or X-linked recessive) with complete penetrance. There were fewer monogenic candidates in the probands with congenital hypopituitarism than those with NFSS (1.3 vs. 2.5 candidate variants/proband, P = 0.024). We did not find any candidate variants (0 of 13 probands) in genes previously reported to explain the phenotype in congenital hypopituitarism, unlike NFSS (8 of 19 probands, P = 0.01).

Conclusion: Our findings provide evidence that the etiology of sporadic congenital hypopituitarism has a major genetic component but may be infrequently monogenic with full penetrance, suggesting a more complex etiology.

Keywords: combined pituitary hormone deficiencies; congenital hypopituitarism; digenic; ectopic posterior pituitary gland; monogenic.