This study used a new approach to fabricate a glucose detection system based on nano-engineered biomaterials. The fabrication steps included strategic synthesis, integration and stabilization of biological and metal nanoparticles in superabsorbent hydrogel gum matrix. The design of the high-performance electrochemical biosensor platform includes copper-micro mesh grid electrode modified with polymer phase comprising of silver nanoparticles surface coroneted with Ceratonia silique locust bean gum (LBG), Tamarindus indica seed-shell nanoparticles and glucose oxidase (GOx). Fundamental assessment of catalytic properties of the nanobiocomposite films on copper grid probe were performed by cyclic voltammetry, amperometry, differential pulse voltammetry. Probes showed good repeatability, reproducibility, selectivity, and long-term stability. The GOx was well-immobilized and stabilized by C. siliqua nano-matrix, with 85% and 98% activity retention when stored at different condiions for 6 month and 3 months, respectively. The fabricated grid-platform exhibited linear response in a wide range of glucose concentration, with detection limit of 1.0 nM (S/N = 3) and sensitivity 38.7 mA nM-1 cm-2. The bionanomaterial-based sensor was successfully applied for ultra-low glucose detection in artificial salivary samples. The designed sensor, perhaps with further modifications, has potential for the next generation of sensing platform in various biological fluids especially for non-invasive glucose detection for diabetic patients.
Keywords: Artificial salivary samples; Copper-micro mesh grid working electrode; Electrochemical glucose biosensor; Parkia biglobosa locust bean gum; Tamarindus indica seed-shell nanoparticles.
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