Chrysophyllum cainito. L alleviates diabetic and complications by playing antioxidant, antiglycation, hypoglycemic roles and the chemical profile analysis

Abstract

Ethnopharmacological relevance: Chrysophyllum cainito L. (C. cainito) is a traditional folk medicine in tropical area which can be an alternative agent for diabetes mellitus. Although the antioxidant and antidiabetic activity of the extracts are reported, little is known on the antiglycation activity and effects on diabetic complications.

Aim of the study: This work was aimed to investigate the chemical profile, antidiabetic, antioxidant activities of C. cainito. Especially, the antiglycation potential as well as the relationships between components and activities were evaluated.

Materials and methods: The content of the primary components (polyphenols, flavonoids, steroids, and triterpenes), antioxidant, and hypoglycemic effects of ethanolic extracts from C. cainito leaves (CCE-1, 2, 3, 4) and stems (CSE-1, 2, 3, 4) were analyzed and detected. The chemical profiles of CCE-2 were characterized by HPLC-Q-TOF-MS/MS. The antiglycation and protection against oxidative stress effects were determined by in vitro assays. Relationship between bioactivities and components was analyzed by principal component analysis (PCA), heatmap analysis, and Pearson correlation analysis.

Results: The composition was diverse between leaves and stem extracts with different activities. CCE-2 possessed the highest DPPH scavenging activity. CSE-2 displayed the highest ABTS scavenging activity and ferric reducing power. While CCE-3 showed the most effective inhibition on α-amylase and α-glucosidase activity (IC₅₀ 4.103 ± 0.332 μg/mL and 0.180 ± 0.006 mg/mL, respectively). PCA analysis showed that the most important variables in PC1 (60.7%) were total polyphenol and antioxidant activities. The hypoglycemic activity and contents of steroids showed important correlation. Advanced glycation end products formation was effectively inhibited by CCE-2 with myricetin 3-O-rhamnoside as the main constituent. CCE-3 displayed the highest protection effect against L02 cell line oxidation damage.

Conclusions: C. cainito leaves might be a promising candidate for antioxidant, hypoglycemic and antiglycation dietary supplement or potential agent against diabetes associated chronic diseases.

Keywords: Antiglycation; Antioxidant; Diabetes; Oxidative stress; Phytochemistry; Polyphenols.