The Effect of Dark Septate Endophytic Fungi on Mahonia oiwakensis

Lei-Chen Lin; Yin-Ling Tan; Wan-Rou Lin; Kuo-Lung Ku; Shang-Tse Ho

doi:10.3390/plants10081723

The Effect of Dark Septate Endophytic Fungi on Mahonia oiwakensis

Plants (Basel). 2021 Aug 20;10(8):1723. doi: 10.3390/plants10081723.

Authors

Lei-Chen Lin¹, Yin-Ling Tan¹, Wan-Rou Lin², Kuo-Lung Ku³, Shang-Tse Ho⁴

Affiliations

¹ Department of Forestry and Natural Resources, National Chiayi University, Chiayi 60004, Taiwan.
² Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute (FIRDI), Hsinchu 30062, Taiwan.
³ Department of Applied Chemistry, National Chiayi University, Chiayi 60004, Taiwan.
⁴ Department of Wood Based Materials and Design, National Chiayi University, Chiayi 60004, Taiwan.

Abstract

This is the first study to discuss the effects of dark septate endophytes (DSE) on the growth promotion and berberine concentration in Mahonia oiwakensis, whose extract (MOE) has been suggested to have potential therapeutic effects against human lung cancer. First, as per phylogenetic analysis, the strains were divided into four groups: CkDB2, CkDB5, MoAL2 and MoAL5. All of these were DSEs, which could form microsclerotia in M. oiwakensis. The growth response experiment revealed that inoculation of the plant with MoAL5 and CkDB5 promoted an increase in the total fresh weight of the seedlings. Chemical composition analysis showed that seedlings inoculated with CkDB5 had the highest berberine concentration. These results showed that some DSEs have the ability to promote growth and induce phytochemical responses in the host plant.

Keywords: Cladophialophora chaetospira; Hymenoscyphus; Mahonia oiwakensis; berberine; dark septate endophyte; growth response.