Antioxidant Activity and Anti-Photoaging Effects on UVA-Irradiated Human Fibroblasts of Rosmarinic Acid Enriched Extract Prepared from Thunbergia laurifolia Leaves

Thanawat Pattananandecha; Sutasinee Apichai; Jakaphun Julsrigival; Malyn Ungsurungsie; Suched Samuhasaneetoo; Pat Chulasiri; Pakakrong Kwankhao; Supaporn Pitiporn; Fumihiko Ogata; Naohito Kawasaki; Chalermpong Saenjum

doi:10.3390/plants10081648

Antioxidant Activity and Anti-Photoaging Effects on UVA-Irradiated Human Fibroblasts of Rosmarinic Acid Enriched Extract Prepared from Thunbergia laurifolia Leaves

Plants (Basel). 2021 Aug 11;10(8):1648. doi: 10.3390/plants10081648.

Authors

Thanawat Pattananandecha^{1

2}, Sutasinee Apichai^{1

2

3}, Jakaphun Julsrigival^{1

2}, Malyn Ungsurungsie^{1

3}, Suched Samuhasaneetoo⁴, Pat Chulasiri⁵, Pakakrong Kwankhao⁶, Supaporn Pitiporn⁶, Fumihiko Ogata⁷, Naohito Kawasaki⁷, Chalermpong Saenjum^{1

2

3}

Affiliations

¹ Cluster of Excellence on Biodiversity Based Economics and Society (B.BES-CMU), Chiang Mai University, Chiang Mai 50200, Thailand.
² Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.
³ Center of Excellence for Innovation in Analytical Science and Technology, Department of Chemistry (I-ANALY-S-T), Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
⁴ Department of Food Technology, Faculty of Engineering and Industrial Technology, Silpakorn University, Nakhon Pathom 73000, Thailand.
⁵ Department of Orthopaedic, Rajavithi Hospital, Ratchathewi, Bangkok 10400, Thailand.
⁶ Chophraya Abhaibhubejhr Hospital, Prachin Anuson Rd., Tha Ngam, Mueang Prachinburi District, Prachin Buri 25000, Thailand.
⁷ Faculty of Pharmacy, Kindai University, 3-4-1 Kowakae, Higashi-Osaka 577-8502, Japan.

Abstract

The current study investigated the inhibiting effect on reactive oxygen species (ROS), reactive nitrogen species (RNS), and matrix metalloproteinase-1 (MMP-1) production in a cell-based study of standardized rosmarinic acid enriched extract (SRAEE) prepared from Thunbergia laurifolia leaves. HPLC chromatogram revealed that rosmarinic acid is a major component in prepared SRAEE, followed by caffeic acid. SRAEE exhibited antioxidant activity both in vitro and cell-based studies. SRAEE showed scavenging effects on nitric oxide and superoxide anion and inhibition effects on lipid peroxidation in vitro. SRAEE also inhibited ROS and MMP-1 production in normal human dermal fibroblast cells induced by H₂O₂ and UVA, respectively, without exerted cytotoxicity. Additionally, collagen degradation was protected by SRAEE induced by UVA. Nitric oxide and inducible nitric oxide synthase (iNOS) productions were also inhibited by SRAEE in RAW264.7 mouse macrophage cells induced by combined lipopolysaccharide (LPS)-interferon-γ (IFN-γ). The results indicated that SRAEE is a potential candidate as a natural pharmaceutical active ingredient for cosmeceutical product application.

Keywords: MMP-1; Thunbergia laurifolia leaf; anti-inflammatory; antioxidants; rosmarinic acid.