Molecular and functional characterization of mitochondrial manganese superoxide dismutase from Macrobrachium rosenbergii during bacterial infection

Yanan Li; Fanbin Zhan; Fenglin Li; Zhijie Lu; Zizheng Xu; Youcheng Yang; Fei Shi; Lijuan Zhao; Zhendong Qin; Li Lin

doi:10.1016/j.fsi.2021.08.025

Molecular and functional characterization of mitochondrial manganese superoxide dismutase from Macrobrachium rosenbergii during bacterial infection

Fish Shellfish Immunol. 2021 Nov:118:94-101. doi: 10.1016/j.fsi.2021.08.025. Epub 2021 Aug 24.

Authors

Yanan Li¹, Fanbin Zhan¹, Fenglin Li¹, Zhijie Lu¹, Zizheng Xu¹, Youcheng Yang¹, Fei Shi¹, Lijuan Zhao¹, Zhendong Qin², Li Lin³

Affiliations

¹ Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong Province, 510222, China.
² Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong Province, 510222, China. Electronic address: qinzhendongsc@163.com.
³ Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center, Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong Province, 510222, China. Electronic address: linli@zhku.edu.cn.

PMID: 34450271
DOI: 10.1016/j.fsi.2021.08.025

Abstract

Superoxide dismutases (SODs) are the main antioxidant enzymes involved in alleviating oxidative stress. Although mitochondrial manganese SOD (mMnSOD) has been reported to be correlated with the immune response in crustaceans, its biological properties and role in the immune response remain unclear. Here, we cloned the Macrobrachium rosenbergii mMnSOD (MrmMnSOD), analyzed its activity and expression pattern under Staphylococcus aureus and Vibrio parahaemolyticus infection, and further explored its possible mechanism during antibacterial immune response. The results showed that both enzyme activity and the expression of MrmMnSOD were significantly up-regulated by bacterial infection. MrmMnSOD knockdown made the prawn susceptible to Vibrio infection, which increased the mortality rate and the number of bacteria in haemocytes. The bacterial agglutination assay confirmed that MrmMnSOD decreases bacterial abundance via agglutination. Overall, this work identified antibacterial function of MrmMnSOD in the immune response. In addition to contributing to immunological theory, these findings aid disease prevention and control in crustacean aquaculture.

Keywords: Antibacterial immunity; Innate immunity; M. rosenbergii; MnSOD; Oxidative stress.

MeSH terms

Animals
Anti-Bacterial Agents
Aquaculture
Bacteria
Bacterial Infections*
Palaemonidae* / genetics
Superoxide Dismutase / genetics

Substances

Anti-Bacterial Agents
Superoxide Dismutase