OsEDM2L mediates m6 A of EAT1 transcript for proper alternative splicing and polyadenylation regulating rice tapetal degradation

Abstract

N⁶ -methyladenosine (m⁶ A) modification affects the post-transcriptional regulation of eukaryotic gene expression, but the underlying mechanisms and their effects in plants remain largely unknown. Here, we report that the N⁶ -adenine methyltransferase-like domain-containing protein ENHANCED DOWNY MILDEW 2-LIKE (OsEDM2L) is essential for rice (Oryza sativa L.) anther development. The osedm2l knockout mutant showed delayed tapetal programmed cell death (PCD) and defective pollen development. OsEDM2L interacts with the transcription factors basic helix-loop-helix 142 and TAPETUM DEGENERATION RETARDATION to regulate the expression of ETERNAL TAPETUM 1 (EAT1), a positive regulator of tapetal PCD. Mutation of OsEDM2L altered the transcriptomic m⁶ A landscape, and caused a distinct m⁶ A modification of the EAT1 transcript leading to dysregulation of its alternative splicing and polyadenylation, followed by suppression of the EAT1 target genes OsAP25 and OsAP37 for tapetal PCD. Therefore, OsEDM2L is indispensable for proper messenger RNA m⁶ A modification in rice anther development.

Keywords: N6-methyladenosine (m6A); alternative polyadenylation (APA); alternative splicing (AS); programmed cell death (PCD).