Identification of Resistance Genes Using Diagnostic R-Gene Enrichment Sequencing (dRenSeq)

Miles Armstrong; Ingo Hein

doi:10.1007/978-1-0716-1609-3_10

Identification of Resistance Genes Using Diagnostic R-Gene Enrichment Sequencing (dRenSeq)

Methods Mol Biol. 2021:2354:213-219. doi: 10.1007/978-1-0716-1609-3_10.

Authors

Miles Armstrong¹, Ingo Hein^{2

3}

Affiliations

¹ Division of Plant Sciences, School of Life Science, University of Dundee (at the James Hutton Institute), Dundee, UK.
² Division of Plant Sciences, School of Life Science, University of Dundee (at the James Hutton Institute), Dundee, UK. i.hein@dundee.ac.uk.
³ The James Hutton Institute, CMS, Invergowrie, Dundee, UK. i.hein@dundee.ac.uk.

PMID: 34448162
DOI: 10.1007/978-1-0716-1609-3_10

Abstract

Diagnostic Resistance gene enrichment Sequencing (dRenSeq) is a method for tracking and verifying the integrity of multiple functional NLR genes in complex genetic backgrounds. Traditionally, these tasks have been addressed by laborious and time-consuming PCR-based approaches or, where cognate avirulence genes are available, effector recognition studies. Both these approaches are superseded by dRenSeq in terms of cost-effectiveness, efficiency, and accuracy.

Keywords: Coverage calculation; Enrichment sequencing; Illumina; Plant resistance; Read mapping.

Identification of Resistance Genes Using Diagnostic R-Gene Enrichment Sequencing (dRenSeq)

Authors

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Abstract

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