The complete mitochondrial genome of Bombus filchnerae (Hymenoptera: Apidae) and phylogenetic analysis

Feng Zhou; Guoyan Zhang; Liyuan Yao; Peng Yu; Lingyun Chen; Yingzhi Ning

doi:10.1080/23802359.2021.1966329

The complete mitochondrial genome of Bombus filchnerae (Hymenoptera: Apidae) and phylogenetic analysis

Mitochondrial DNA B Resour. 2021 Aug 24;6(9):2727-2729. doi: 10.1080/23802359.2021.1966329. eCollection 2021.

Authors

Feng Zhou¹, Guoyan Zhang¹, Liyuan Yao¹, Peng Yu¹, Lingyun Chen¹, Yingzhi Ning¹

Affiliation

¹ College of Life Science, Northwest Normal University, Lanzhou, China.

Abstract

Here, we report the complete mitochondrial genome (mitogenome) of Bombus filchnerae (Hymenoptera: Apidae). The genome size of B. filchnerae was 18,553 bp with 88.7% A + T content, containing 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes, and an AT-rich control region (D-loop). tRNA rearrangement was observed in this mitochondrial genome when compared to those of other bumblebee species (e.g. Bombus breviceps and Bombus asiaticus). All the 13 PCGs initiated with typical ATN codons. Among them, 11 PCGs terminated with TAA, only nad4 and nad5 with incomplete stop codon TA and T, respectively. All the 22 tRNAs can be folded into typical cloverleaf structure, except for trnS1, whose dihydrouridine (DHU) arm forms a simple loop. The phylogenetic analysis based on the concatenated nucleotide sequences of all 13 PCGs indicated that B. filchnerae showed the closest relationship with Bombus pascuorum, forming a mono clade of the subgenus Thoracobombus, with well-resolved relationships among nine Bombus subgenera.

Keywords: Bumblebee; mitochondrial genome; phylogenetic relationship.

Grants and funding

This work was supported by the Project for Enhancing the Innovation Capability of Higher School in Gansu Province [No. 2020B-093]; Natural Science Foundation of Gansu Province [No. 20JR10RA103]; The Project for Enhancing the Research Capability of Young Teachers in Northwest Normal University [No. NWNU-LKQN2019-22]; Youth Science and Technology Talents Lifting Project Foundation of Gansu Province.