Non-enzymatic Transformation of Aflatoxin B1 by Pseudomonas geniculata m29

Yuanyuan Yao; Xian Shu; Dongdong Wang; Wenjie Kan; Pengfei Su; Hao Hu; Xu Chen; Dacheng Wang; Shengwei Huang; Lifang Wu

doi:10.3389/fmicb.2021.724103

Non-enzymatic Transformation of Aflatoxin B₁ by Pseudomonas geniculata m29

Front Microbiol. 2021 Aug 10:12:724103. doi: 10.3389/fmicb.2021.724103. eCollection 2021.

Authors

Yuanyuan Yao^{1

2}, Xian Shu^{1

2}, Dongdong Wang¹, Wenjie Kan¹, Pengfei Su¹, Hao Hu¹, Xu Chen¹, Dacheng Wang¹, Shengwei Huang¹, Lifang Wu^{1

2}

Affiliations

¹ Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, China.
² School of Life Sciences, University of Science and Technology of China, Hefei, China.

Abstract

Aflatoxin B₁ (AFB₁) is the most harmful mycotoxin produced by filamentous fungi and presents a serious threat to human and animal health. Therefore, it is essential to protect humans and animals from AFB₁-induced acute and chronic toxicity. In this study, Pseudomonas strain m29 having a high efficiency of AFB₁ transformation was isolated from soil. The transformation ratio by m29 was more than 97% within 24 h, and the optimum temperature for transformation was 37°C. Moreover, the AFB₁ transforming activity was mainly attributed to the cell-free supernatant of strain m29. The metabolite that plays a crucial role in AFB₁ transformation is likely 1,2-dimethylhydrazine or 1,1-dimethylhydrazine, as identified by GC-MS and LC-MS analysis. AFB₁ was transformed into a product with molecular formula C₁₇H₁₄O₇. To the best of our knowledge, this is the first study of non-enzymatic AFB₁ transformation by bacteria. Importantly, this AFB₁ transformation mechanism could be universal to various microorganisms.

Keywords: LC-MS analysis; Pseudomonas geniculata m29; aflatoxin B1; non-enzymatic; transformation.