Radiolabeling and Preliminary Evaluation of 99mTc-Labeled DNA Cube Nanoparticles as Potential Tracers for SPECT Imaging

Xiaoyan Duan; Yiri Du; Chunmei Wang; Zhenfeng Zhao; Chao Li; Jianbo Li

doi:10.2147/IJN.S325791

Radiolabeling and Preliminary Evaluation of ^99mTc-Labeled DNA Cube Nanoparticles as Potential Tracers for SPECT Imaging

Int J Nanomedicine. 2021 Aug 20:16:5665-5673. doi: 10.2147/IJN.S325791. eCollection 2021.

Authors

Xiaoyan Duan¹, Yiri Du¹, Chunmei Wang^{1

2}, Zhenfeng Zhao^{1

2}, Chao Li¹, Jianbo Li^{1

2}

Affiliations

¹ Inner Mongolia Medical University Affiliated Hospital, Hohhot, 010050, People's Republic of China.
² Inner Mongolia Key Laboratory of Molecular Imaging, Hohhot, 010050, People's Republic of China.

Abstract

Purpose: DNA nanostructures, with the advantages of structural designability and spatial addressability, have shown a great potential in the field of drug delivery and bio-medicine. Herein, we aimed to prepare technetium-99m radiolabeled DNA cube nanoparticles (^99mTc-DCN) and expect to build a DCN-based drug carrier and nuclear medicine imaging platform.

Methods: DCN could be readily assembled with 6 designed DNA oligonucleotides at an equal mole ratio in a single annealing procedure. ^99mTc-MAG3-ssDNA (A20) was obtained by labeling MAG3-ssDNA (A20) with technetium-99m by using a stannous chloride reduction method. ^99mTc-DCN was prepared by hybridize DCN with side chains (T20-DCN) with ^99mTc-MAG3-ssDNA (A20). The biodistribution study and SPECT/CT imaging were conducted on KM mice.

Results: DCN was successfully assembled, and as-prepared DCN was characterized by native polyacrylamide gel electrophoresis, atomic force microscope and fluorescence resonance energy transfer. The size of DCN was about 5 nm. The radiolabeling yield of ^99mTc-MAG3-ssDNA (A20) was approximately 90% by radio thin-layer chromatography. T20-DCN mixed with ^99mTc-MAG3-ssDNA (A20) in PBS could generate ^99mTc-DCN upon hybridization. The retention time (RT) of ^99mTc-MAG3-ssDNA (A20) was at ~22 min, and the RT of as-prepared ^99mTc-DCN was at ~12 min by radio-HPLC. The results from biodistribution study and SPECT/CT imaging showed that a significant proportion of DCNs were metabolized through the liver and kidney. Intestine exhibited a relatively indicative signal as well, which might be explained by the enterohepatic circulation of DCN via the liver and gallbladder.

Conclusion: We have successfully prepared ^99mTc-DCN as a SPECT/CT imaging probe via the side-chain hybridization strategy. The probe was metabolized mainly by the liver and excreted primarily to the bladder. Due to the superior properties of DNA cube nanoparticles, we believe DCN may potentially be translated into a preclinical setting for diagnosis and treatment of cancer-related diseases.

Keywords: DNA cube nanoparticles; SPECT/CT imaging; Tc-99m labeling; biodistribution.

MeSH terms

Animals
DNA
Mice
Nanoparticles*
Technetium*
Tissue Distribution
Tomography, Emission-Computed, Single-Photon

Substances

Technetium
DNA

Grants and funding

National Natural Science Foundation of China (No. 82060324), Natural Science Foundation of Inner Mongolia (2018LH08044) and Science and Technology Program of Inner Mongolia (2019GG106).