Objective: This study retrospectively analyzed an outbreak of dengue fever in Puyang of Henan province in 2019, in order to find the sources of infection. Methods: Dengue virus IgM/IgG and NS1 antigen were tested by colloidal gold method. E gene was amplified by PCR. MegaX was used for sequences alignment to construct evolutionary distance trees. Results: After clinical and laboratory confirmation, there were 81 cases of dengue fever, 17 of which were imported case who were local farmers and worked in Combadia and Thailand, and 64 of which were indigenous cases. The E gene alignment results showed that the pathogen of this epidemic was Vietnamese 1 and highly homologous with the Vietnamese strain. After the local outbreak, dengue virus E gene developed a nucleotide site mutation which can be steadily transmission. Conclusion: The dengue fever outbreak in Puyang was a local outbreak caused by dengue virus type 1, which was associated with imported cases. Gene sequencing showed that the imported pathogen had a relatively stable and transmissible nucleotide mutation after the local epidemic.
目的： 分析河南省濮阳市2019年8月份的一起登革热暴发疫情。 方法： 利用胶体金法检测登革病毒IgM/IgG抗体和NS1抗原，利用PCR方法扩增E基因，并进行基因测序，利用MegaX软件进行E基因编码蛋白序列的比对分析，构建进化树。 结果： 经过临床确诊和实验室确诊，共有81例登革热病例，其中17例为当地农民到柬埔寨和泰国务工的输入性病例，64例为本土病例。E基因编码的蛋白序列比对分析结果表明，该起疫情的病原为登革热病毒1型，与越南株高度同源。本土疫情发生后，登革病毒E基因发生了核苷酸位点突变，突变位点可稳定传播。 结论： 濮阳本起登革热暴发疫情系与国外输入病例相关联的一起由登革1型病毒引起的本地暴发，基因测序显示输入性病原在本地流行后发生了较为稳定且可传播的核苷酸突变。.