Establishment of a novel human iPSC line (YCMi003-A) from a patient with dilated cardiomyopathy carrying genetic variant LMNA p.Asp364His

Stem Cell Res. 2021 Oct:56:102508. doi: 10.1016/j.scr.2021.102508. Epub 2021 Aug 19.

Abstract

Cardiac laminopathy caused by mutations in the LMNA gene are common and highly penetrant with a poor prognosis. We have generated a novel human induced pluripotent stem cell(iPSC) lines YCMi003-A from a patient with dilated cardiomyopathy associated with genetic variant LMNA c.1090G > C; p.Asp364His. We reprogrammed patient-specific peripheral blood mononuclear cells using five episomal vectors Oct4, Sox2, Lin28, L-Myc, and Klf4. The reported iPSC line would be a useful model for in vitro modeling of cardiac laminopathy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cardiomyopathy, Dilated* / genetics
  • Cell Differentiation
  • Humans
  • Induced Pluripotent Stem Cells*
  • Kruppel-Like Factor 4
  • Lamin Type A / genetics
  • Leukocytes, Mononuclear
  • Mutation

Substances

  • KLF4 protein, human
  • Kruppel-Like Factor 4
  • LMNA protein, human
  • Lamin Type A