A method to establish a chronic restraint stress mouse model with colorectal cancer xenografts

Shiyun Shao; Zhaozhou Zhang; Yingru Zhang; Ru Jia; Yiyang Zhao; Qi Li; Yan Wang

doi:10.1016/j.mex.2021.101304

A method to establish a chronic restraint stress mouse model with colorectal cancer xenografts

MethodsX. 2021 Mar 10:8:101304. doi: 10.1016/j.mex.2021.101304. eCollection 2021.

Authors

Shiyun Shao^{1

2}, Zhaozhou Zhang^{1

2}, Yingru Zhang^{1

2}, Ru Jia¹, Yiyang Zhao¹, Qi Li^{1

2}, Yan Wang¹

Affiliations

¹ Department of Medical Oncology, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
² Academy of Integrative Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.

Abstract

Colorectal cancer (CRC) remains one of the most common clinical cancers of digestive tract. Recently, a large number of researches have shown that chronic stress can actively participate in the development of CRC. The proposed method successfully established the model of chronic stress mouse model with colorectal cancer.•Chronic restraint stress (CRS) was used to establish chronic stress model.•CRS was combined with a colorectal cancer xenografts model.•Behavioural tests and tumour growth were used to evaluate model construction.

Keywords: Chronic stress; Colorectal cancer; Depression.