Two species of loaches, Misgurus anguillicaudatus and Burbatula toni were collected from 28 localities of Hokkaido to detect trematode metacercariae. Among 829 M. anguillicaudatus, 577 (69.6%) were found to be infected with metacercariae, while 9 (1.6%) out of 549 B. toni were infected with metacercariae. Adult flukes were recovered from the intestine of mice and rats or dogs orally administered with metacercariae. Based on the morphological examination of adult specimens, Echinostoma hortense was identified. Metacercariae of E. hortense were found on 507 (60.8%) of M. anguillicaudatus, but not detected on B. toni. The average number of metacercariae of E. hortense per one loach was 3.6. They were obtained from all 22 localities where M. anguillicaudatus was collected. A small number of metacercariae of Metagonimus sp. and Diplostomum sp. were found on M. anguillicaudatus and B. toni. The worms belonging to these genera were not able to be identified by the present study.