A review of research progress of heterotrophic nitrification and aerobic denitrification microorganisms (HNADMs)

Abstract

Traditional nitrogen removal relies on the autotrophic nitrification and anaerobic denitrification process. In the system, autotrophic microorganisms achieve nitrification under aerobic condition and heterotrophic microorganisms complete the denitrification in anaerobic condition. As the two types of microorganisms have different tolerance on oxygen concentration, nitrification and denitrification are normally set in two compartments for high nitrogen removal. Therefore, large land occupying is required. In fact, there is a special type of microorganism called heterotrophic nitrification & aerobic denitrification microorganisms (HNADMs) which can oxidize ammonium nitrogen, and perform denitrification in the presence of oxygen. HNADMs have been reported in many environments. It was found that HNADMs could simultaneously achieve nitrification and denitrification. In addition, some HNADMs not only have the ability to remove nitrogen, but also have the ability to remove phosphorus. It suggests that HNADMs have great potential for pollution removal from wastewater. So far, individual work on single strain was carried out. Comprehensive summary of the HNADMs would provide a better picture for understanding and directing its application. In this paper, the studies related on HNADMs were reviewed. The nitrogen metabolism pathway of HNADMs was summarized. The impact of pH, DO, carbon source, and C/N on HNADMs growth and metabolism were discussed. In addition, the extracellular polymeric substance (EPS) production, quorum sensing (QS) secretion and P removal by HNADMs were displayed.

Keywords: Extracellular polymeric substance; Nitrogen assimilation; P removal; Quorum sensing; Simultaneous nitrification and denitrification.