A solid-liquid triboelectric nanogenerator (TENG) has attracted increasing research interest in relation to the development of regeneration energy based on water resources. The output of solid-liquid TENG remains unsolved, however, because of the low voltage output that impedes wide applications. To this end, in this work we developed a miniaturized microfluidic channel-based TENG device for highly efficient conversion of energy from the transport of a water droplet to an electrical output. We investigated an optimized design in a triboelectric material, the droplet transport and the electrostatic induction layer to provide a high voltage output and stable energy harvesting. The optimized device demonstrated maximum voltage amplitude 102 mV with an ultralow liquid consumption, 0.36 [Formula: see text], resulting in sample-energy conversion 283.33 mV/ [Formula: see text]. This novel device is expected potentially to address the limitations imposed by sample consumption in energy harvesting in the future.