Differences in the spectroscopic characteristics of wetland dissolved organic matter binding with Fe3+, Cu2+, Cd2+, Cr3+ and Zn2

Abstract

Understanding of the binding characteristics of wetland dissolved organic matter (DOM) and different metals is important for the quantitative assessment of the environmental behavior of metals in wetlands. In this study, different types of spectroscopy including ultraviolet-visible absorption, Fourier transform infrared, and fluorescence spectroscopy was used to investigate the binding characteristics of Fe³⁺, Cu²⁺, Cr³⁺, Cd²⁺, and Zn²⁺ with DOM from wetland water. Differential absorption spectra identified binding sites for these five metals in this wetland DOM at 210 nm, 280 nm, 335 nm, and > 400 nm regions. The low binding capacity of DOM in this wetland with Cd and Zn indicated that the toxicity and environmental effects of these metals in this wetland warrant further study. The calculated △EEM combined with fluorescence regional integration (FRI) analysis clearly revealed that Fe and Cu preferred to bind with humic-like DOM while Cd and Zn preferred to bind with protein-like DOM in this wetland. △EEM successfully demonstrated the characteristics of DOM complexing with different metals and could be a compelling tool in evaluating metal-DOM interactions. In addition, 2D-FTIR-COS identified the binding sites and the dynamic processes of binding at the functional group level. Metals preferentially bind with the CO, CO functional group, and then binds to the OH functional group. This study revealed that different DOM components will facilitate the migration of different metals in the environment and provided new slights into an improved understanding of migration and transformation of metals in aquatic environments.

Keywords: Binding; Dissolved organic matter; Metals; Spectroscopy.